Mork-Jansson Astrid Elisabeth, Gargano Daniela, Kmiec Karol, Furnes Clemens, Shevela Dmitriy, Eichacker Lutz Andreas
Center for Organelle Research, University of Stavanger, Stavanger, Norway.
Center for Organelle Research, University of Stavanger, Stavanger, Norway; Department of Chemistry, Chemical Biological Centre (KBC), Umeå University, Sweden.
FEBS Lett. 2015 Oct 7;589(20 Pt B):3064-70. doi: 10.1016/j.febslet.2015.08.023. Epub 2015 Aug 29.
The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8 nM) is favored relative to homodimerization (431±59 nM). Interaction of Lil3.2 with chlorophyll a (231±49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana.
双螺旋类光捕获（Lil）蛋白Lil3属于光合膜叶绿素结合光捕获蛋白家族。已证明其在四吡咯合成和香叶基香叶醇还原酶稳定化方面具有功能。Lil蛋白含有叶绿素a/b结合基序；然而，尚未证实其与叶绿素的结合。我们发现拟南芥中的Lil3.2与Lil3.1形成异二聚体并结合叶绿素。相对于同二聚体化（431±59 nM），Lil3.2异二聚体化（25±7.8 nM）更为有利。Lil3.2与叶绿素a的相互作用（231±49 nM）表明，在拟南芥中，异二聚体化先于叶绿素结合。
