Wareth Gamal, Melzer Falk, Weise Christoph, Neubauer Heinrich, Roesler Uwe, Murugaiyan Jayaseelan
Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Berlin, Germany ; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Jena, Germany.
Data Brief. 2015 Jul 31;4:587-90. doi: 10.1016/j.dib.2015.07.029. eCollection 2015 Sep.
Here, we provide the dataset associated with our research article on comprehensive screening of Brucella immunoreactive proteins using sera of naturally infected hosts published in Biochemical and Biophysical Research Communications Wareth et al., 2015 [1]. Whole-cell protein extracts were prepared from Brucella abortus and Brucella melitensis, separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently western blotting was carried out using sera from bovines (cows and buffaloes) and small ruminants (goats and sheep). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository [2] with the dataset identifiers PXD001270 and DOI:10.6019/PXD001270.
在此，我们提供了与我们发表在《生化与生物物理研究通讯》（Wareth等人，2015年[1]）上的关于使用自然感染宿主血清对布鲁氏菌免疫反应蛋白进行全面筛选的研究文章相关的数据集。从流产布鲁氏菌和马尔他布鲁氏菌制备全细胞蛋白提取物，使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分离，随后使用牛（奶牛和水牛）和小反刍动物（山羊和绵羊）的血清进行蛋白质印迹分析。质谱蛋白质组学数据已通过PRIDE合作伙伴存储库[2]存入蛋白质组交换联盟（http://proteomecentral.proteomexchange.org），数据集标识符为PXD001270和DOI:10.6019/PXD001270。
