Poetsch Ansgar, Marchesini María Inés
Center for Marine and Molecular Biotechnology, QNLM, Qingdao 266237, China.
College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China.
Proteomes. 2020 Apr 22;8(2):8. doi: 10.3390/proteomes8020008.
spp. are Gram negative intracellular bacteria responsible for brucellosis, a worldwide distributed zoonosis. A prominent aspect of the Brucella life cycle is its ability to invade, survive and multiply within host cells. Comprehensive approaches, such as proteomics, have aided in unravelling the molecular mechanisms underlying Brucella pathogenesis. Technological and methodological advancements such as increased instrument performance and multiplexed quantification have broadened the range of proteome studies, enabling new and improved analyses, providing deeper and more accurate proteome coverage. Indeed, proteomics has demonstrated its contribution to key research questions in Brucella biology, i.e., immunodominant proteins, host-cell interaction, stress response, antibiotic targets and resistance, protein secretion. Here, we review the proteomics of Brucella with a focus on more recent works and novel findings, ranging from reconfiguration of the intracellular bacterial proteome and studies on proteomic profiles of Brucella infected tissues, to the identification of Brucella extracellular proteins with putative roles in cell signaling and pathogenesis. In conclusion, proteomics has yielded copious new candidates and hypotheses that require future verification. It is expected that proteomics will continue to be an invaluable tool for Brucella and applications will further extend to the currently ill-explored aspects including, among others, protein processing and post-translational modification.
布鲁氏菌属是革兰氏阴性胞内细菌，可引发布鲁氏菌病，这是一种在全球范围内传播的人畜共患病。布鲁氏菌生命周期的一个显著特征是其能够在宿主细胞内侵袭、存活和繁殖。蛋白质组学等综合方法有助于揭示布鲁氏菌发病机制的分子机制。仪器性能提升和多重定量等技术和方法的进步拓宽了蛋白质组研究的范围，实现了新的、改进的分析，提供了更深入、更准确的蛋白质组覆盖。事实上，蛋白质组学已证明其对布鲁氏菌生物学关键研究问题的贡献，即免疫显性蛋白、宿主细胞相互作用、应激反应、抗生素靶点与耐药性、蛋白质分泌。在此，我们回顾布鲁氏菌的蛋白质组学，重点关注最新研究成果和新发现，从细胞内细菌蛋白质组的重新配置以及布鲁氏菌感染组织蛋白质组图谱的研究，到鉴定在细胞信号传导和发病机制中具有假定作用的布鲁氏菌细胞外蛋白。总之，蛋白质组学产生了大量需要未来验证 的新候选物和假设。预计蛋白质组学将继续成为研究布鲁氏菌的宝贵工具，其应用还将进一步扩展到目前研究较少的方面，包括蛋白质加工和翻译后修饰等。
