Kloc M, Miller M, Carrasco A E, Eastman E, Etkin L
Department of Molecular Genetics, University of Texas MD Anderson Cancer Center, Houston 77030.
Development. 1989 Dec;107(4):899-907. doi: 10.1242/dev.107.4.899.
We have attempted to analyze the function of a maternal mRNA xlgv7 which is distributed as an animal-vegetal gradient in stage 6 oocytes using a combination of antisense oligodeoxynucleotide injection into oocytes followed by in vitro maturation and fertilization. Injection of 20 ng of the antisense oligodeoxynucleotide resulted in the destruction of the xlgv7 mRNA to undetectable levels. Upon maturation and fertilization the resulting embryos develop with no specific defects suggesting that the maternal store of xlgv7 in stage 6 oocytes is not required and that the embryo can develop solely with the maternal store of the xlgv7 protein. Also, these results demonstrate the feasibility of this approach in destroying a specific maternal RNA and assaying its effect on development.
我们尝试分析一种母体mRNA xlgv7的功能，该mRNA在6期卵母细胞中呈动物-植物梯度分布。我们采用了向卵母细胞注射反义寡脱氧核苷酸，随后进行体外成熟和受精的方法。注射20 ng反义寡脱氧核苷酸会导致xlgv7 mRNA被破坏至无法检测的水平。成熟和受精后，产生的胚胎发育没有特定缺陷，这表明6期卵母细胞中xlgv7的母体储存并非必需；胚胎仅依靠xlgv7蛋白的母体储存就能发育。此外，这些结果证明了这种方法在破坏特定母体RNA并检测其对发育影响方面的可行性。
