Schmid Michael, Steinbeisser Herbert, Epperlein Hans-Henning, Trendelenburg Michael F, Lipps Hans J
Medizinisch-Naturwissenschaftliches Forschungszentrum, Universität Tübingen, Ob dem Himmelreich 7, W-7400, Tübingen, Germany.
Deutsches Krebsforschungszentrum, Institut für Experimentelle Pathologie, Heidelberg, Germany.
Rouxs Arch Dev Biol. 1992 Oct;201(6):340-345. doi: 10.1007/BF00365121.
An expression vector was constructed containing the entire bovine papilloma virus (BPV-1) genome and part of the a-actin gene of Xenopus laevis cloned in the antisense orientation into the neomycin resistance gene under the control of the herpes simplex virus (HSV) thymidine kinase (TK) promoter. When this vector is microinjected into X. laevis embryos it replicates extrachromosomally, at least up to the tadpole stage, and a fusion RNA is synthesized after the mid blastula transition (MBT). The expression of the antisense gene results in a morphological abnormality of somites demonstrating that antisense RNA generated by an episomal replicating expression vector can inhibit the expression of a selected gene during early embryogenesis of X. laevis.
构建了一个表达载体，其包含完整的牛乳头瘤病毒（BPV-1）基因组以及非洲爪蟾α-肌动蛋白基因的一部分，该部分以反义方向克隆到在单纯疱疹病毒（HSV）胸苷激酶（TK）启动子控制下的新霉素抗性基因中。当将此载体显微注射到非洲爪蟾胚胎中时，它会在染色体外复制，至少直到蝌蚪阶段，并且在囊胚中期转变（MBT）后会合成融合RNA。反义基因的表达导致体节出现形态异常，这表明由游离复制表达载体产生的反义RNA可以在非洲爪蟾早期胚胎发育过程中抑制所选基因的表达。
