Hira Tohru, Yahagi Asuka, Nishimura Saki, Sakaino Masayoshi, Yamashita Takatoshi, Hara Hiroshi
Research Faculty of Agriculture, Hokkaido University , Sapporo 060-8589, Japan.
Graduate School of Agriculture, Hokkaido University , Sapporo 060-8589, Japan.
J Agric Food Chem. 2015 Sep 23;63(37):8177-81. doi: 10.1021/acs.jafc.5b03126. Epub 2015 Sep 8.
We recently demonstrated that a diunsaturated aldehyde, trans,trans-2,4-decadienal (2,4-decadienal), potently stimulated secretion of cholecystokinin in the enteroendocrine cell line. Gut hormones such as cholecystokinin and serotonin play critical roles in reducing postprandial gastric emptying. In the present study, we first demonstrated that oral administration of 2,4-decadienal (50-100 mg/kg) reduced gastric emptying rate in rats, assessed by both the acetaminophen absorption test and the phenol red recovery method. In contrast, saturated aldehyde, alcohol, and fatty acids having the same chain length as 2,4-decadienal did not affect the gastric emptying rate. Duodenal administration of 2,4-decadienal potently reduced gastric emptying rate, but intraperitoneal administration did not. Furthermore, the gastric inhibitory effect of 2,4-decadienal was attenuated by treatment with a serotonin receptor antagonist. These results demonstrated that 2,4-decadienal in the small intestinal lumen has a potent inhibitory effect on gastric emptying, possibly through stimulation of the serotonin-producing enteroendocrine cells.
我们最近证明，一种二不饱和醛，反式，反式-2,4-癸二烯醛（2,4-癸二烯醛），能有效刺激肠内分泌细胞系中胆囊收缩素的分泌。胆囊收缩素和5-羟色胺等胃肠激素在降低餐后胃排空方面发挥着关键作用。在本研究中，我们首先证明，通过对乙酰氨基酚吸收试验和酚红回收法评估，口服2,4-癸二烯醛（50-100 mg/kg）可降低大鼠的胃排空率。相比之下，与2,4-癸二烯醛具有相同链长的饱和醛、醇和脂肪酸对胃排空率没有影响。十二指肠给药2,4-癸二烯醛可有效降低胃排空率，但腹腔注射则无此作用。此外，用5-羟色胺受体拮抗剂处理可减弱2,4-癸二烯醛对胃的抑制作用。这些结果表明，小肠腔内的2,4-癸二烯醛对胃排空具有强大的抑制作用，可能是通过刺激产生5-羟色胺的肠内分泌细胞实现的。
