Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia; Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia.
Plant Physiol Biochem. 2015 Nov;96:296-300. doi: 10.1016/j.plaphy.2015.08.014. Epub 2015 Aug 24.
Basal stem rot is one of the major diseases of oil palm (Elaies guineensis Jacq.) caused by pathogenic Ganoderma species. Trichoderma and mycorrhizae were proposed to be able to reduce the disease severity. However, their roles in improving oil palm defence system by possibly inducing defence-related genes in the host are not well characterized. To better understand that, transcript profiles of eleven putative defence-related cDNAs in the roots of oil palm inoculated with Trichoderma harzianum T32 and mycorrhizae at different time points were studied. Transcripts encoding putative Bowman-Birk protease inhibitor (EgBBI2) and defensin (EgDFS) increased more than 2 fold in mycorrhizae-treated roots at 6 weeks post inoculation (wpi) compared to those in controls. Transcripts encoding putative dehydrin (EgDHN), glycine-rich RNA binding protein (EgGRRBP), isoflavone reductase (EgIFR), type 2 ribosome inactivating protein (EgT2RIP), and EgDFS increased in the oil palm roots treated with T. harzianum at 6 and/or 12 wpi compared to those in the controls. Some of these genes were also expressed in oil palm roots treated with Ganoderma boninense. This study provides an insight of some defence-related genes induced by Trichoderma and mycorrhizae, and their roles as potential agents to boost the plant defence system.
基腐病是油棕（Elaies guineensis Jacq.）的主要病害之一，由病原性灵芝属物种引起。拟青霉属和菌根被认为能够降低疾病的严重程度。然而，它们通过可能诱导宿主防御相关基因在改善油棕防御系统中的作用尚未得到很好的描述。为了更好地理解这一点，研究了接种拟青霉属（Trichoderma harzianum T32）和菌根后的油棕根中 11 种推测的防御相关 cDNA 的转录谱，在不同时间点。在接种后 6 周（wpi）的菌根处理根中，编码推测的 Bowman-Birk 蛋白酶抑制剂（EgBBI2）和防御素（EgDFS）的转录物比对照中的转录物增加了 2 倍以上。在接种后 6 和/或 12 wpi 的油棕根中，编码推测的脱水素（EgDHN）、甘氨酸丰富的 RNA 结合蛋白（EgGRRBP）、异黄酮还原酶（EgIFR）、2 型核糖体失活蛋白（EgT2RIP）和 EgDFS 的转录物增加，与对照相比。其中一些基因也在油棕根中表达 被波尼恩青霉处理。本研究提供了一些由拟青霉属和菌根诱导的防御相关基因的见解，以及它们作为增强植物防御系统的潜在因子的作用。
