Naher Laila, Tan Soon Guan, Ho Chai Ling, Yusuf Umi Kalsom, Ahmad Siti Hazar, Abdullah Faridah
Department of Biology, Faculty of Science, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia.
ScientificWorldJournal. 2012;2012:647504. doi: 10.1100/2012/647504. Epub 2012 Aug 2.
Basal stem rot (BSR) disease caused by the fungus Ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agent Trichoderma harzianum can protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensis Jacq.) chitinases encoding EgCHI1, EgCHI2, and EgCHI3 at 2, 5, and 8 weeks inoculation were measured in oil palm leaves from plants treated with G. boninense or T. harzianum alone or both.
The five-month-old oil palm seedlings were treated with Gano-wood blocks inoculum and trichomulch. Expression of EgCHI1, EgCHI2, and EgCHI3 in treated leaves tissue was determined by real-time PCR.
Oil palm chitinases were not strongly expressed in oil palm leaves of plants treated with G. boninense alone compared to other treatments. Throughout the 8-week experiment, expression of EgCHI1 increased more than 3-fold in leaves of plants treated with T. harzianum and G. boninense when compared to those of control and other treated plants.
The data illustrated that chitinase cDNA expression varied depending on tissue and the type of treatment.
由真菌博宁灵芝引起的油棕基部干腐病（BSR）是影响油棕最严重的病害；这是因为该病害难以早期检测。生物防治剂哈茨木霉仅能在病害早期保护植株。在本研究中，测定了单独用博宁灵芝或哈茨木霉处理或二者都处理的油棕植株叶片中，编码EgCHI1、EgCHI2和EgCHI3的三种油棕（油棕属）几丁质酶在接种后2周、5周和8周时的表达水平。
用含博宁灵芝木块的接种物和木霉覆盖物处理5月龄油棕幼苗。通过实时PCR测定处理叶片组织中EgCHI1、EgCHI2和EgCHI3的表达。
与其他处理相比，单独用博宁灵芝处理的油棕植株叶片中油棕几丁质酶表达不强。在整个8周的实验中，与对照和其他处理植株相比，用哈茨木霉和博宁灵芝处理的植株叶片中EgCHI1的表达增加了3倍多。
数据表明几丁质酶cDNA表达因组织和处理类型而异。
