School of Chemical and Biological Engineering, Seoul National University , Seoul 151-744, Republic of Korea.
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology , Yuseong, Daejeon 305-600, Republic of Korea.
Nano Lett. 2015 Oct 14;15(10):6559-67. doi: 10.1021/acs.nanolett.5b02286. Epub 2015 Sep 24.
Human sensory-mimicking systems, such as electronic brains, tongues, skin, and ears, have been promoted for use in improving social welfare. However, no significant achievements have been made in mimicking the human nose due to the complexity of olfactory sensory neurons. Combinational coding of human olfactory receptors (hORs) is essential for odorant discrimination in mixtures, and the development of hOR-combined multiplexed systems has progressed slowly. Here, we report the first demonstration of an artificial multiplexed superbioelectronic nose (MSB-nose) that mimics the human olfactory sensory system, leading to high-performance odorant discriminatory ability in mixtures. Specifically, portable MSB-noses were constructed using highly uniform graphene micropatterns (GMs) that were conjugated with two different hORs, which were employed as transducers in a liquid-ion gated field-effect transistor (FET). Field-induced signals from the MSB-nose were monitored and provided high sensitivity and selectivity toward target odorants (minimum detectable level: 0.1 fM). More importantly, the potential of the MSB-nose as a tool to encode hOR combinations was demonstrated using principal component analysis.
人类感官模拟系统，如电子大脑、舌头、皮肤和耳朵，已被推广用于改善社会福利。然而，由于嗅觉感觉神经元的复杂性，人类鼻子的模拟一直没有取得重大进展。人类嗅觉受体 (hOR) 的组合编码对于混合物中的气味辨别至关重要，而 hOR 组合的多路复用系统的发展进展缓慢。在这里，我们报告了第一个模仿人类嗅觉感觉系统的人工多路复用超生物电子鼻 (MSB-nose) 的演示，该系统在混合物中具有高性能的气味辨别能力。具体来说，使用高度均匀的石墨烯微图案 (GM) 构建了便携式 MSB-nose，该图案与两种不同的 hOR 结合，作为液体离子门控场效应晶体管 (FET) 的换能器。从 MSB-nose 监测到的场诱导信号对目标气味具有高灵敏度和选择性（最小可检测水平：0.1 fM）。更重要的是，通过主成分分析证明了 MSB-nose 作为编码 hOR 组合的工具的潜力。
