Zheng Fang, Jiang Ze-bo, Zhang Xian, Hu Jin-ping, Li Si-ming, Zhao Jin, Zeng Xing
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1798-802.
To study the effect of the combined administration of different doses of Glycyrrhizae Radix et Rhizoma and Atractylodis Macrocephalae Rhizoma on the proliferation of DFMO-treated intestinal epithelial cells (IEC-6) and p53, p21 mRNA and protein expressions, in order to define the molecular basis for the effect of the combined administration of different doses of Glycyrrhizae Radix et Rhizoma and Atractylodis Macrocephalae Rhizoma on the cell proliferation. The effect of the drugs on the cell division rate and cell cycle of IEC-6 cells was detected by FCM. Quantitative Real-time PCR (qRT-PCR) was used to analyze the effect of the drugs on mRNA of p2l and p53 related to IEC-6 proliferation. Western blot was used to analyze the effect of the drugs on p2l and p53 protein expressions of IEC-6 cells. Atractylodis Macrocephalae Rhizoma could increase p53, p21 mRNA and proteins expression in DFMO-treated IEC-6 cells. The combined administration of different ratios of Atractylodis Macrocephalae Rhizoma and Glycyrrhizae Radix et Rhizoma could significantly down-regulate Atractylodis Macrocephalae Rhizoma's effect on p53, p21 mRNA and proteins expression in DFMO-treated IEC-6 cells and promote the proliferation of IEC-6 cells. The combined administration of Atractylodis Macrocephalae Rhizoma and Glycyrrhizae Radix et Rhizoma could down-regulate Atractylodis Macrocephalae Rhizoma's effect on DFMO-treated intestinal epithelial cells (IEC-6).
研究不同剂量甘草与白术联合应用对二氟甲基鸟氨酸（DFMO）处理的肠上皮细胞（IEC-6）增殖及p53、p21 mRNA和蛋白表达的影响，以明确不同剂量甘草与白术联合应用对细胞增殖影响的分子基础。采用流式细胞术（FCM）检测药物对IEC-6细胞分裂率及细胞周期的影响。运用实时定量聚合酶链反应（qRT-PCR）分析药物对与IEC-6增殖相关的p21和p53 mRNA的影响。采用蛋白质免疫印迹法（Western blot）分析药物对IEC-6细胞p21和p53蛋白表达的影响。白术可增加DFMO处理的IEC-6细胞中p53、p21 mRNA及蛋白表达。不同比例白术与甘草联合应用可显著下调白术对DFMO处理的IEC-6细胞中p53、p21 mRNA及蛋白表达的影响，并促进IEC-6细胞增殖。白术与甘草联合应用可下调白术对DFMO处理的肠上皮细胞（IEC-6）的影响。
