[白术质量评价的高效液相色谱指纹图谱研究]
[HPLC fingerprint study for the quality evaluation of atractylodis macrocephalae rhizoma].
作者信息
Chen Xiang-Dong, Zhang Guang-Da, Lan Xiao-Yong, Liu Fa-Jin, Wei Mei
机构信息
Guangdong Yifang Pharmaceutical Co., Ltd., Foshan 528244, China.
出版信息
Zhong Yao Cai. 2013 Feb;36(2):208-12.
OBJECTIVE
To establish an HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma (warm dried).
METHODS
Took methanol extract of Atractylodis Macrocephalae Rhizoma as sample by ultrasonic processing, HPLC analysis was carried out on Sinochrom ODS-BP (4.6 mm x 250 mm, 5 microm) chromatographic column with mobile phase of acetonitrile (A)-water (B), gradient elution with a flow at 1.0 mL/min, ultraviolet detection wavelength at 242 nm and column temperature at 30 degrees C.
RESULTS
An HPLC fingerprint analysis method for Atractylodis Macrocephalae Rhizoma was developed. Eight common peaks were obtained and three peaks were identified.
CONCLUSION
The method is accurate and credible which can be used for quality control of Atractylodis Macrocephalae Rhizoma (warm dried).
目的
建立一种白术（烘）的高效液相色谱指纹图谱分析方法。
方法
采用超声处理后的白术甲醇提取物作为样品，在中色谱ODS-BP（4.6mm×250mm，5μm）色谱柱上进行高效液相色谱分析，流动相为乙腈（A）-水（B），梯度洗脱，流速为1.0mL/min，紫外检测波长为242nm，柱温为30℃。
结果
建立了白术的高效液相色谱指纹图谱分析方法。得到8个共有峰，鉴定出3个峰。
结论
该方法准确可靠，可用于白术（烘）的质量控制。
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