Vigo Germán B, Giacoboni Gabriela I, Gagetti Paula S, Pasterán Fernando G, Corso Alejandra C
Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
Rev Argent Microbiol. 2015 Jul-Sep;47(3):206-11. doi: 10.1016/j.ram.2015.06.002. Epub 2015 Aug 29.
Twenty-eight strains isolated from dog clinical samples identified as Staphylococcus pseudintermedius by mass spectrometry (MALDI-TOF) were studied to assess antimicrobial susceptibility by the diffusion method and clonal relationship by pulsed field gel electrophoresis (PFGE). Methicillin resistance (3/28 isolates; 10,7%) was evaluated by mecA PCR. Fifteen strains (53.6%) were resistant to at least one of the antibiotics tested, and eleven of them (39.3%) showed multiple resistance (3 or more antimicrobial families). Eleven isolates (39.3%) were resistant to erythromycin due to the presence of ribosomal methylase ermB, whereas clindamycin inducible resistance was not detected. Twenty-seven (27) clonal types were differentiated by PFGE, suggesting high clonal diversity. We emphasize that the finding of multiresistant S. psedintermedius strains is an emerging problem to be considered in veterinary diagnostic laboratory treatment of canine infections and in public health settings.
对通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）鉴定为中间型假葡萄球菌的28株从犬临床样本中分离出的菌株，采用扩散法评估其抗菌药敏性，并通过脉冲场凝胶电泳（PFGE）分析其克隆关系。通过mecA基因PCR检测耐甲氧西林情况（3/28株分离菌；10.7%）。15株（53.6%）对至少一种测试抗生素耐药，其中11株（39.3%）表现出多重耐药（对3种或更多抗菌药物类别耐药）。11株分离菌（39.3%）因存在核糖体甲基化酶ermB而对红霉素耐药，未检测到克林霉素诱导型耐药。通过PFGE区分出27种克隆型，表明克隆多样性高。我们强调，耐多药中间型假葡萄球菌菌株的发现是兽医诊断实验室在犬类感染治疗以及公共卫生环境中需要考虑的一个新出现的问题。
