Department of Veterinary Medicine, University of Bari, Strada p.le per Casamassima Km 3, Valenzano-Bari 70010, Italy.
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark.
Vet Microbiol. 2017 Nov;211:103-106. doi: 10.1016/j.vetmic.2017.09.015. Epub 2017 Oct 12.
In the last decade there has been a rapid global spread of methicillin-resistant Staphylococcus pseudintermedius (MRSP) clones displaying multidrug resistance in dogs. We investigated prevalence, antimicrobial susceptibility and clonal distribution of MRSP isolated from clinical canine samples between during 2011-2014. Following species identification by nuc PCR, MRSP were confirmed by the presence of mecA and characterized by antimicrobial susceptibility testing, Pulsed Field Gel Electrophoresis (PFGE), SCCmec typing, and Multi-Locus Sequence Typing (MLST) of a few isolates having distinct PFGE profiles. Both the MRSP isolation frequency in the 175 samples tested (12%) and the prevalence of methicillin resistance amongst the 63S. pseudintermedius isolates (33%) were high compared to a previous study in Italy. Sequence type (ST)71 carrying SCCmec type II-III, described as the epidemic European MRSP clone, accounted for approximately half of the isolates. The remaining isolates belonged to ST410-SCCmec type II-III, ST258-SCCmec type IV and other three clones associated with SCCmec type IV (ST261, ST290 and ST477). MRSP were consistently resistant to potentiated sulfonamides, and more frequently to clindamycin, ciprofloxacin and doxycycline than methicillin-susceptible isolates. Gentamicin was the only antibiotic showing good in vitro activity on all MRSP with 20 of the 21 isolates being susceptible. Results confirm a high prevalence of MRSP amongst clinical samples in Italy, revealing the emergence of new clones other than ST71, such as ST258, ST410, ST261, ST290 and ST477, here describe for the first time. Implementation of antimicrobial stewardship and surveillance programmes are required to prevent the emergence of new MRSP clones and reducing transmission in small animal practice.
在过去的十年中，耐甲氧西林金黄色葡萄球菌（MRSP）克隆在狗中迅速传播，这些克隆具有多种药物耐药性。我们调查了 2011 年至 2014 年期间从临床犬样本中分离的 MRSP 的流行率、抗菌药物敏感性和克隆分布。通过 nuc PCR 进行种属鉴定后，通过 mecA 的存在确认 MRSP，并通过抗菌药物敏感性试验、脉冲场凝胶电泳（PFGE）、SCCmec 分型和少数具有独特 PFGE 图谱的分离株的多位点序列分型（MLST）进行特征描述。与意大利之前的一项研究相比，在测试的 175 个样本中，MRSP 的分离频率（12%）和 63 个金黄色葡萄球菌中间亚种分离株中的耐甲氧西林率（33%）均较高。携带 SCCmec 类型 II-III 的 ST71 型（称为流行的欧洲 MRSP 克隆）约占分离株的一半。其余分离株属于 ST410-SCCmec 类型 II-III、ST258-SCCmec 类型 IV 和另外三个与 SCCmec 类型 IV 相关的克隆（ST261、ST290 和 ST477）。MRSP 对增效磺胺类药物始终具有耐药性，并且比甲氧西林敏感分离株更频繁地对克林霉素、环丙沙星和强力霉素耐药。庆大霉素是唯一对所有 MRSP 均显示出良好体外活性的抗生素，21 株中有 20 株敏感。结果证实，意大利临床样本中 MRSP 的流行率较高，显示出除 ST71 以外的新克隆的出现，例如 ST258、ST410、ST261、ST290 和 ST477，这些克隆首次在这里描述。需要实施抗菌药物管理和监测计划，以防止新的 MRSP 克隆出现并减少小动物诊疗中的传播。
