使用不同方法测定瘤胃未降解蛋白的肠道消化率及相关性分析
Measurement of the Intestinal Digestibility of Rumen Undegraded Protein Using Different Methods and Correlation Analysis.
作者信息
Wang Y, Zhang Y G, Liu Xiaolan, Kopparapu N K, Xin Hangshu, Liu J, Guo Jianhua
机构信息
College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China ; Animal Science and Technology Institute, Northeast Agriculture University, Harbin 150030, China .
Animal Science and Technology Institute, Northeast Agriculture University, Harbin 150030, China .
出版信息
Asian-Australas J Anim Sci. 2015 Oct;28(10):1454-64. doi: 10.5713/ajas.15.0085.
Four methods were adopted, including the mobile nylon bag (MNB) method, modified three-step in vitro (MTS) method, original three-step in vitro (OTS) method, and acid detergent insoluble nitrogen (ADIN) estimating method, to evaluate the intestinal digestibility of rumen undegradable protein (DRUP) of 10 types of concentrates and 7 types of roughages. After correlation analysis to determine the DRUP values using the MNB, MTS, OTS, and ADIN methods, the study aimed to find out appropriate methods to replace the MNB method due to its disadvantages such as high price, long time period, and use of a duodenal T-fistula. Three dairy cows with a permanent ruminal fistula and duodenal T-fistula were used in a single-factor experimental design. The results showed that the determined DRUP values using the MNB method for soybean meal, cottonseed meal, rapeseed meal, sunflower meal, corn germ meal, corn, rice bran, barley, wheat bran, corn fiber feed, Alfalfa (Zhao dong), Alfalfa (Long mu 801), Alfalfa (Long mu 803), grass (North), Grass (Inner Mongolia), corn silage and corn straw were 98.13%, 87.37%, 88.47%, 82.60%, 75.40%, 93.23%, 69.27%, 91.27%, 72.37%, 79.03%, 66.72%, 68.64%, 73.57%, 50.47%, 51.52%, 54.05%, and 43.84%, respectively. The coefficient of determination (R (2) = 0.964) of the results between the MTS method and the MNB method was higher than that (R (2) = 0.942) between the OTS method and the MNB method. The coefficient of determination of the DRUP values of the concentrates among the in vitro method (including the MTS and OTS methods) and the MNB method was higher than that of the roughage. There was a weak correlation between the determined DRUP values in concentrates obtained from the ADIN method and those from the MNB method, and there was a significant correlation (p<0.01) between the determined DRUP values of the roughage obtained from the MNB method and those obtained from ADIN method. The DRUP values were significantly correlated with the nutritional ingredients of the feeds. The regression equation was DRUP =100.5566+0.4169CP - 0.4344SP - 0.7102NDF - 0.7950EE (R (2) = 0.8668, p<0.01; CP, crude protein; SP, soluble protein; NDF, neutral detergent fiber; EE, ether extract). It was concluded that both the MTS method and the OTS may suitable to replace the MNB method for determining the DRUP values and the former method was more effective. Only the ADIN method could be used to predict the values of the roughages but conventional nutritional ingredients were available for all of the samples' DRUP.
采用移动尼龙袋法（MNB）、改良三步体外法（MTS）、原始三步体外法（OTS）和酸性洗涤不溶性氮（ADIN）估测法4种方法，对10种精饲料和7种粗饲料的瘤胃不可降解蛋白（DRUP）的小肠消化率进行评估。在对采用MNB、MTS、OTS和ADIN法测定的DRUP值进行相关性分析后，鉴于MNB法存在价格高、耗时且需使用十二指肠T型瘘管等缺点，本研究旨在找出合适的方法来替代MNB法。采用单因素试验设计，选用3头装有永久性瘤胃瘘管和十二指肠T型瘘管的奶牛。结果表明，采用MNB法测定豆粕、棉籽粕、菜籽粕、葵花粕、玉米胚芽粕、玉米、米糠、大麦、麸皮、玉米纤维饲料、苜蓿（肇东）、苜蓿（龙牧801）、苜蓿（龙牧803）、羊草（北疆）、羊草（内蒙古）、玉米青贮和玉米秸秆的DRUP值分别为98.13%、87.37%、88.47%、82.60%、75.40%、93.23%、69.27%、91.27%、72.37%、79.03%、66.72%、68.64%、73.57%、50.47%、51.52%、54.05%和43.84%。MTS法与MNB法测定结果的决定系数（R² = 0.964）高于OTS法与MNB法测定结果的决定系数（R² = 0.942）。体外法（包括MTS和OTS法）与MNB法测定精饲料DRUP值的决定系数高于粗饲料。ADIN法测定精饲料DRUP值与MNB法测定结果之间相关性较弱，而MNB法测定粗饲料DRUP值与ADIN法测定结果之间存在显著相关性（p<0.0¹）。DRUP值与饲料营养成分显著相关。回归方程为DRUP =100.5566 + 0.4169CP - 0.4344SP - 0.7102NDF - 0.7950EE（R² = 0.8668，p<0.0¹；CP，粗蛋白；SP，可溶性蛋白；NDF，中性洗涤纤维；EE，乙醚浸出物）。研究得出结论，MTS法和OTS法均可用于替代MNB法测定DRUP值，且前者更有效。只有ADIN法可用于预测粗饲料的DRUP值，但所有样品的DRUP均可采用常规营养成分进行预测。
Zotero 插件