Bozorgzadeh Somayyeh, Hamidi Hassan, Ortiz Roberto, Ludwig Roland, Gorton Lo
Department of Analytical Chemistry/Biochemistry and Structural Biology, Lund University, SE-22100 Lund, Sweden.
Phys Chem Chem Phys. 2015 Oct 7;17(37):24157-65. doi: 10.1039/c5cp03812j. Epub 2015 Sep 1.
In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs-MWCNTs and PdNPs-MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (Imax) and the apparent Michaelis-Menten constants (K) for the different PcCDH modified electrodes were calculated by fitting the data to the Michaelis-Menten equation and compared. The sensitivity towards lactose was 3.07 and 3.28 μA mM(-1) at the PcCDH/PtNPs-MWCNTs/SPGE and PcCDH/PdNPs-MWCNTs/SPGE electrodes, respectively, which were higher than those measured at the PcCDH/MWCNTs/SPGE (2.60 μA mM(-1)) and PcCDH/SPGE (0.92 μA mM(-1)). The modified electrodes were additionally tested as bioanodes for biofuel cell applications.
在本工作中，通过一种简单的热分解方法将铂和钯纳米颗粒（PtNPs和PdNPs）修饰在多壁碳纳米管（MWCNTs）表面。将制备的纳米杂化物PtNPs-MWCNTs和PdNPs-MWCNTs浇铸在光谱石墨电极表面，然后将黄孢原毛平革菌纤维二糖脱氢酶（PcCDH）吸附在修饰层上。使用流动注射安培法和循环伏安法研究了PcCDH与纳米结构修饰电极之间的直接电子转移。通过将数据拟合到米氏方程计算并比较了不同PcCDH修饰电极的最大电流响应（Imax）和表观米氏常数（K）。在PcCDH/PtNPs-MWCNTs/SPGE和PcCDH/PdNPs-MWCNTs/SPGE电极上对乳糖的灵敏度分别为3.07和3.28 μA mM(-1)，高于在PcCDH/MWCNTs/SPGE（2.60 μA mM(-1)）和PcCDH/SPGE（0.92 μA mM(-1)）上测得的灵敏度。还对修饰电极作为生物燃料电池应用的生物阳极进行了测试。
