Friesen William L, Schultz Brian J, Destino Joel F, Alivio Theodore E G, Steet Joseph R, Banerjee Sarbajit, Wood Troy D
Department of Chemistry, Natural Sciences Complex, University at Buffalo, Buffalo, NY, 14260-3000, USA.
Department of Chemistry, Texas A & M University, College Station, TX, 77843-3255, USA.
J Am Soc Mass Spectrom. 2015 Nov;26(11):1963-6. doi: 10.1007/s13361-015-1243-6. Epub 2015 Sep 1.
Here, a matrix using two-dimensional (2D) graphene is demonstrated for the first time in the context of MALDI IMS using a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. Although graphene flakes have been used previously in MALDI, it is described here how a single 2D layer of graphene is applied directly on top of rat brain sections and soybean leaves. Several classes of molecules are desorbed and ionized off of the surface of the tissues examined using 2D graphene, with minimal background interference from the matrix. Moreover, no solvents are employed in application of 2D graphene, eliminating the potential for analyte diffusion in liquid droplets during matrix application. Because 2D graphene is an elemental form of carbon, an additional advantage is its high compatibility with the long duration needed for many IMS experiments. Graphical Abstract ᅟ.
在此，首次展示了在使用傅里叶变换离子回旋共振（FT-ICR）质谱仪的基质辅助激光解吸电离成像质谱（MALDI IMS）背景下使用二维（2D）石墨烯的基质。尽管石墨烯薄片此前已用于MALDI，但本文描述了如何将单个二维石墨烯层直接应用于大鼠脑切片和大豆叶片之上。使用二维石墨烯从所检查的组织表面解吸并电离了几类分子，基质产生的背景干扰极小。此外，在二维石墨烯的应用中不使用溶剂，消除了在基质应用过程中分析物在液滴中扩散的可能性。由于二维石墨烯是碳的一种元素形式，另一个优点是它与许多成像质谱实验所需的长时间具有高度兼容性。图形摘要ᅟ 。
