维持和监测无菌啮齿动物的特定微生物群状态。
Maintaining and Monitoring the Defined Microbiota Status of Gnotobiotic Rodents.
作者信息
Nicklas Werner, Keubler Lydia, Bleich André
机构信息
Werner Nicklas, Dr. med. vet., DipECLAM, is head of the Microbiological Diagnostics of the German Cancer Research Center in Heidelberg, Germany. Lydia Keubler, PhD, is German board certified veterinarian and research associate and André Bleich, PhD, DipECLAM, is director of the Institute for Laboratory Animal Science and Central Animal Facility of the Hannover Medical School in Hannover, Germany.
出版信息
ILAR J. 2015;56(2):241-9. doi: 10.1093/ilar/ilv029.
Gnotobiotic (germfree, defined colonized) rodents have become powerful tools to advance our understanding of the host-microbiome relationship. However, the maintenance and ultimately the monitoring of gnotobiotic rodents is a critical, labor-intensive, and costly process (e.g., sterility, not absence of specific pathogens, must be demonstrated in germfree animals). Here, we provide information on the housing and maintenance of gnotobiotic animals, elucidate prophylactic measurements to avoid contamination, and make specific recommendations for sampling procedures, sampling frequencies, and test methods.
无菌（无特定病原体、已知菌群定植）啮齿动物已成为推动我们理解宿主与微生物组关系的有力工具。然而，无菌啮齿动物的饲养以及最终的监测是一个关键、劳动密集且成本高昂的过程（例如，必须证明无菌动物是无菌的，而非仅仅没有特定病原体）。在此，我们提供有关无菌动物饲养和维护的信息，阐明预防污染的措施，并针对采样程序、采样频率和检测方法提出具体建议。
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