Ganewatta Mitra S, Miller Kristen P, Singleton S Parker, Mehrpouya-Bahrami Pegah, Chen Yung P, Yan Yi, Nagarkatti Mitzi, Nagarkatti Prakash, Decho Alan W, Tang Chuanbing
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine , Columbia, South Carolina 29209, United States.
Department of Applied Chemistry, School of Science, Northwestern Polytechnical University , Xi'an 710129, China.
Biomacromolecules. 2015 Oct 12;16(10):3336-44. doi: 10.1021/acs.biomac.5b01005. Epub 2015 Sep 10.
We report antibacterial, antibiofilm, and biocompatible properties of surface-immobilized, quaternary ammonium-containing, resin acid-derived compounds and polycations that are known to be efficient antimicrobial agents with minimum toxicities to mammalian cells. Surface immobilization was carried out by the employment of two robust, efficient chemical methods: Copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition click reaction, and surface-initiated atom transfer radical polymerization. Antibacterial and antibiofilm activities against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were strong. Hemolysis assays and the growth of human dermal fibroblasts on the modified surfaces evidenced their biocompatibility. We demonstrate that the grafting of quaternary ammonium-decorated abietic acid compounds and polymers from surfaces enables the incorporation of renewable biomass in an effective manner to combat bacteria and biofilm formation in biomedical applications.
我们报道了表面固定的、含季铵的、树脂酸衍生化合物和聚阳离子的抗菌、抗生物膜和生物相容性特性，这些化合物已知是高效的抗菌剂，对哺乳动物细胞的毒性最小。通过两种强大、有效的化学方法进行表面固定：铜催化的叠氮化物-炔烃1,3-偶极环加成点击反应和表面引发的原子转移自由基聚合。对革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌的抗菌和抗生物膜活性很强。溶血试验以及人真皮成纤维细胞在改性表面上的生长证明了它们的生物相容性。我们证明，从表面接枝季铵修饰的枞酸化合物和聚合物能够以有效的方式纳入可再生生物质，以对抗生物医学应用中的细菌和生物膜形成。
