Romanowski Maciej, Kłoda Karolina, Milczarek Sławomir, Pawlik Andrzej, Domański Leszek, Safranow Krzysztof, Ciechanowski Kazimierz
Department of General and Transplantation Surgery, Pomeranian Medical University, Szczcein, Poland.
Clinical Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland.
Ann Transplant. 2015 Sep 1;20:506-11. doi: 10.12659/AOT.893885.
BACKGROUND Allograft inflammatory factor (AIF-1) is a recently studied cytoplasmic protein encoded by the AIF1 gene in humans. The main function of AIF-1 is in the chronic inflammatory process. The aim of this study was to examine the impact of the rs2269475: C>T, rs2736182: G>A, and rs2259571: A>C AIF1 gene polymorphisms on long-term kidney allograft function and graft loss after kidney transplantation. MATERIAL AND METHODS The study enrolled 269 white kidney allograft recipients (165 males, 104 females, mean age 47.63±12.96 years, after transplantation performed between 2000 and 2006). The rs2269475: C>T, rs2736182: G>A, and rs2259571: A>C AIF1 gene polymorphisms were genotyped with TaqMan genotyping assays using a 7500 FAST Real-Time PCR System (Applied Biosystems, USA). RESULTS Creatinine concentrations at 12, 24, 36, 48, and 60 months after transplantation did not differ significantly between the studied genotypes of AIF1 polymorphisms. Cox regression analysis showed no statistically significant associations between the risk of graft loss/return to dialysis and the examined polymorphisms. CONCLUSIONS The results of our study suggest that the AIF1 gene polymorphisms have no influence on long-term kidney allograft function.
背景 同种异体移植炎症因子（AIF-1）是一种最近研究的细胞质蛋白，由人类AIF1基因编码。AIF-1的主要功能参与慢性炎症过程。本研究的目的是检测AIF1基因多态性rs2269475：C>T、rs2736182：G>A和rs2259571：A>C对肾移植后长期肾移植功能和移植肾丢失的影响。
材料与方法 本研究纳入了269名白人肾移植受者（165名男性，104名女性，平均年龄47.63±12.96岁，于2000年至2006年期间进行移植）。使用7500 FAST实时PCR系统（美国应用生物系统公司）的TaqMan基因分型检测法对AIF1基因多态性rs2269475：C>T、rs27三千六百一十八二：G>A和rs2259571：A>C进行基因分型。
结果 在AIF1多态性的研究基因型之间，移植后12、24、36、48和60个月时的肌酐浓度无显著差异。Cox回归分析显示，移植肾丢失/恢复透析风险与所检测的多态性之间无统计学显著关联。
结论 我们的研究结果表明，AIF1基因多态性对长期肾移植功能没有影响。
