Mendez National Institute of Transplantation, Los Angeles, CA, United States; Saint Vincent Medical Center, Los Angeles, CA, United States; Western University of Health Sciences, Pomona, CA, United States.
Hum Immunol. 2013 Oct;74(10):1386-91. doi: 10.1016/j.humimm.2013.06.017. Epub 2013 Jun 15.
Cyclooxygenase-2 (COX-2) alleles have been associated with allograft outcomes in kidney transplant recipients; however, these alleles may be in linkage with other genes. Human allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein and is produced by macrophages. Its synthesis is regulated by several cytokines, including interferon gamma. We investigated whether polymorphisms of gene encoding COX-2 and AIF-1 were associated with allograft outcomes among Hispanic renal transplant recipients (RTRs). A total of 527 de novo RTRs of Hispanic ethnicity were included in this study transplanted at St. Vincent Medical Center (SVMC) during 2000-2009. Patients were genotyped for the following: COX-2 (-1195C>T rs689466, intron 6 rs2066826) and AlF1 (rs2269475). Analysis of the results showed that COX-2-1195 CC genotype (OR=1.92, CI%=1.00-3.67, p=0.04) were more frequent, but COX-2-1195 CT genotype was less frequent in kidney allograft acute rejection in comparison with control group (OR=0.59, CI%=0.38-0.91, p=0.017). The genetic variant TT/CT of the AIF-1 gene was associated with a lower risk of rejection (OR=0.63, CI%=0.41-0.98, p=0.038). No association of COX-2 (rs2066826) was observed with allograft rejection. We are unable to find statistically significant association between COX-2 and AIF-1 gene polymorphisms and allograft survival. The -1195C>T in the COX-2 promoter and AIF-1 gene polymorphisms could be a potential predictor of allograft rejection in our Hispanic kidney transplant recipients.
环氧化酶-2 (COX-2) 等位基因与肾移植受者的移植物结局有关；然而，这些等位基因可能与其他基因连锁。人类同种异体炎症因子-1 (AIF-1) 是一种细胞质蛋白，由巨噬细胞产生。其合成受多种细胞因子的调节，包括干扰素 γ。我们研究了编码 COX-2 和 AIF-1 的基因多态性是否与西班牙裔肾移植受者 (RTR) 的移植物结局有关。本研究共纳入 527 例 2000-2009 年在圣文森特医疗中心 (SVMC) 接受首次肾移植的西班牙裔 RTR。对患者进行 COX-2(-1195C>T rs689466，内含子 6 rs2066826) 和 AIF-1(rs2269475) 基因的多态性分析。结果分析显示，COX-2-1195CC 基因型 (OR=1.92, CI%=1.00-3.67, p=0.04) 更为常见，但 COX-2-1195CT 基因型在肾移植急性排斥反应中较对照组更为少见 (OR=0.59, CI%=0.38-0.91, p=0.017)。AIF-1 基因的 TT/CT 遗传变异与较低的排斥风险相关 (OR=0.63, CI%=0.41-0.98, p=0.038)。COX-2(rs2066826) 与移植物排斥无关。我们未能发现 COX-2 和 AIF-1 基因多态性与移植物存活之间存在统计学显著关联。COX-2 启动子中的-1195C>T 和 AIF-1 基因多态性可能是我们西班牙裔肾移植受者移植排斥的潜在预测因子。
