Pak Theodore R, Altman Deena R, Attie Oliver, Sebra Robert, Hamula Camille L, Lewis Martha, Deikus Gintaras, Newman Leah C, Fang Gang, Hand Jonathan, Patel Gopi, Wallach Fran, Schadt Eric E, Huprikar Shirish, van Bakel Harm, Kasarskis Andrew, Bashir Ali
Icahn Institute and Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Antimicrob Agents Chemother. 2015 Nov;59(11):7117-20. doi: 10.1128/AAC.01723-15. Epub 2015 Aug 31.
Whole-genome sequences for Stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smeT, a repressor for multidrug efflux operon smeDEF. Along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. Whole-genome sequencing and complete assembly can conclusively identify resistance mechanisms emerging in S. maltophilia strains during clinical therapy.
对一名嗜麦芽窄食单胞菌血流感染患者在左氧氟沙星耐药性产生前后的系列分离株进行了全基因组从头组装测序，结果显示多药外排操纵子smeDEF的阻遏物smeT中有一个单核苷酸变异。与同期5例病例的测序分离株一起，与所有已发表的完整基因组相比，它们表现出相当大的多样性。全基因组测序和完整组装可以最终确定嗜麦芽窄食单胞菌菌株在临床治疗期间出现的耐药机制。
