整合子、SmeABC和SmeDEF外排泵对嗜麦芽窄食单胞菌临床分离株多药耐药性的作用。
Contribution of integrons, and SmeABC and SmeDEF efflux pumps to multidrug resistance in clinical isolates of Stenotrophomonas maltophilia.
作者信息
Chang Lin-Li, Chen Hui-Feng, Chang Chung-Yu, Lee Tsong-Ming, Wu Wen-Jeng
机构信息
Department of Microbiology, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
J Antimicrob Chemother. 2004 Mar;53(3):518-21. doi: 10.1093/jac/dkh094. Epub 2004 Jan 28.
OBJECTIVES
The contribution of integrons and efflux pumps to multidrug resistance in Stenotrophomonas maltophilia was evaluated.
MATERIALS AND METHODS
Ninety-three S. maltophilia clinical isolates were studied. PCR and direct sequencing were used to detect the presence of integrons. Real-time PCR was performed to assess and quantify the expression of the Sme efflux pumps of S. maltophilia.
RESULTS
Class 1 integrons were detected in 22% of clinical isolates and carried cassettes conferring resistance mainly to aminoglycosides and trimethoprim. The small multidrug resistance gene, smr, was found on class 1 integrons in six isolates. Thirty-one percent of the isolates overexpressed the smeDEF gene, as compared with a control strain, and 59% overexpressed the smeABC gene. Extrusion of ciprofloxacin and meropenem was specific to the SmeABC and SmeDEF pumps, respectively.
CONCLUSION
SmeABC and SmeDEF efflux pumps play important roles in resistance of S. maltophilia to ciprofloxacin and meropenem.
目的
评估整合子和外排泵在嗜麦芽窄食单胞菌多重耐药性中的作用。
材料与方法
研究了93株嗜麦芽窄食单胞菌临床分离株。采用聚合酶链反应（PCR）和直接测序法检测整合子的存在。进行实时PCR以评估和定量嗜麦芽窄食单胞菌Sme外排泵的表达。
结果
在22%的临床分离株中检测到1类整合子，其携带的基因盒主要赋予对氨基糖苷类和甲氧苄啶的耐药性。在6株分离株的1类整合子上发现了小多重耐药基因smr。与对照菌株相比，31%的分离株smeDEF基因过度表达，59%的分离株smeABC基因过度表达。环丙沙星和美罗培南的外排分别特异于SmeABC和SmeDEF泵。
结论
SmeABC和SmeDEF外排泵在嗜麦芽窄食单胞菌对环丙沙星和美罗培南的耐药性中起重要作用。
Zotero 插件