Guirelli P M, Angeloni M B, Barbosa B F, Gomes A O, Castro A S, Franco P S, Silva R J, Oliveira J G, Martins-Filho O A, Mineo J R, Ietta F, Ferro E A
Laboratory of Immunophysiology of Reproduction, Department of Histology and Embryology, Federal University of Uberlândia, Av. Pará, 1720, Uberlândia, CEP 38400 902, Brazil.
Laboratory of Biomarkers for Diagnosis and Monitoring, René Rachou Research Center, Oswaldo Cruz Foundation, 30190-002 Belo Horizonte, MG, Brazil.
Placenta. 2015 Oct;36(10):1106-14. doi: 10.1016/j.placenta.2015.08.009. Epub 2015 Aug 21.
The interaction between human extravillous trophoblasts and macrophages has an important role in implantation and placentation. However, any dysfunction in this communication system is associated with pregnancy pitfalls, and a Toxoplasma gondii infection can be a potential problem in this crosstalk. Therefore, the aim of this study was to assess the influence of infected macrophages on cytokine production and the incidence of apoptosis in T. gondii-infected extravillous trophoblast cells.
HTR-8/SVneo cells were treated with supernatant from macrophages infected or not by T. gondii (conditioned medium) in order to analyze apoptosis and cytokine production in comparison to uninfected control conditions.
The IL-6 secretion by HTR-8/SVneo cells increased synergistically by treatment with conditioned medium and T. gondii infection. The apoptosis index of HTR-8/SVneo cells was also upregulated by treatment with conditioned medium and infection. In addition, a low expression of Fas/CD95 and a high soluble FasL release were observed during infection, although no significant change was observed in the proliferation of T. gondii.
The parasite modulates the high apoptosis index in HTR-8/SVneo cells in order to favor its establishment inside its host cells. On the other hand, the conditioned medium from uninfected macrophages restores the apoptosis rates, although the effect of the infection seems to be stronger. In conclusion, our results showed that T. gondii infection in human extravillous trophoblasts is able to modulate the trophoblast-macrophage crosstalk.
人绒毛外滋养层细胞与巨噬细胞之间的相互作用在着床和胎盘形成过程中起着重要作用。然而，这种通讯系统的任何功能障碍都与妊娠并发症相关，而弓形虫感染可能是这种串扰中的一个潜在问题。因此，本研究的目的是评估受感染的巨噬细胞对弓形虫感染的绒毛外滋养层细胞中细胞因子产生和细胞凋亡发生率的影响。
用来自感染或未感染弓形虫的巨噬细胞的上清液（条件培养基）处理HTR-8/SVneo细胞，以便与未感染的对照条件相比分析细胞凋亡和细胞因子产生。
与条件培养基和弓形虫感染协同处理后，HTR-8/SVneo细胞的IL-6分泌增加。用条件培养基处理和感染也上调了HTR-8/SVneo细胞的凋亡指数。此外，在感染期间观察到Fas/CD95低表达和高可溶性FasL释放，尽管弓形虫的增殖未观察到显著变化。
该寄生虫调节HTR-8/SVneo细胞中的高凋亡指数，以便有利于其在宿主细胞内的定植。另一方面，未感染巨噬细胞的条件培养基可恢复凋亡率，尽管感染的影响似乎更强。总之，我们的结果表明，人绒毛外滋养层细胞中的弓形虫感染能够调节滋养层-巨噬细胞串扰。
