Letić-Gavrilović A, Radojicić C, Abe K
Department of Oral Biochemistry, Fukuoka Dental College, Japan.
J Dent Res. 1989 Aug;68(8):1252-5. doi: 10.1177/00220345890680080801.
Functional changes of the T-cell immune system in submandibularectomized (SMx) male mice of CBA/H strain were analyzed three or four weeks after treatment. Thymuses and spleens were weighed and morphologically examined by light microscopy. We tested proliferation rates of thymocytes and splenocytes (stimulated by Con A and PHA) and cytotoxic activity of T lymphocytes in SMx, CBA/H (H-2k) mice by means of the rejection test of allogeneic skin graft (Balb/c, H-2d) and a microcytoxicity to allo-antigens of EL-4 tumor cells (H-2b). Results showed a reduction of the weights of the thymus and spleen. However, there was increased functional capacity of the thymocytes--expressed as increased proliferation rates of the stimulated thymocytes, increased microcytotoxicity to allo-antigens of EL-4 tumor cells in vitro, and a reduction of the survival period of skin allografts in vivo. No morphological changes other than a reduction in both the cortex and medullar of the thymus were observed. The subpopulational changes within the T-cell system because of SMx indicate that the morphological atrophy could be just a consequence of SMx.
在处理后的三到四周，对CBA/H品系的下颌下腺切除（SMx）雄性小鼠的T细胞免疫系统的功能变化进行了分析。称量胸腺和脾脏的重量，并通过光学显微镜进行形态学检查。我们通过同种异体皮肤移植（Balb/c，H-2d）的排斥试验和对EL-4肿瘤细胞（H-2b）同种抗原的微量细胞毒性试验，测试了SMx、CBA/H（H-2k）小鼠胸腺细胞和脾细胞的增殖率（由刀豆蛋白A和植物血凝素刺激）以及T淋巴细胞的细胞毒性活性。结果显示胸腺和脾脏重量减轻。然而，胸腺细胞的功能能力增强——表现为受刺激胸腺细胞的增殖率增加、体外对EL-4肿瘤细胞同种抗原的微量细胞毒性增加以及体内皮肤同种异体移植存活期缩短。除了胸腺皮质和髓质均减少外，未观察到其他形态学变化。由于SMx导致的T细胞系统内亚群变化表明，形态学萎缩可能只是SMx的结果。
