Reinthaler F F, Mascher F, Sixl W, Enayat U, Marth E
J Diarrhoeal Dis Res. 1989 Sep-Dec;7(3-4):89-91.
Concentrated stool samples from 560 children, aged from two weeks to 10 years, from Idukki District, southern India, were examined to detect Cryptosporidium oocysts, using a phenol-auramin stain and fluorescent microscopy. Smears of stool samples were also stained by a modified Ziehl-Neelsen method and were examined by light-microscopy. One sample was collected from each of 266 children with diarrhoea and 294 age-matched children without diarrhoea. Cryptosporidium oocysts were detected in 4.5% of all stools, in 6% of diarrhoeal stools and in 3% of stools from healthy controls. Results of the study show that Cryptosporidium infection is common in children with diarrhoea in southern India, and there is also a high frequency of asymptomatic cyst-passers in this area.
对来自印度南部伊杜基地区的560名年龄在两周至10岁之间儿童的浓缩粪便样本进行检测,以使用酚-金胺染色和荧光显微镜检测隐孢子虫卵囊。粪便样本涂片也采用改良齐-尼氏法染色,并通过光学显微镜检查。从266名腹泻儿童和294名年龄匹配的无腹泻儿童中各采集一份样本。在所有粪便样本中,4.5%检测到隐孢子虫卵囊,腹泻粪便样本中为6%,健康对照儿童的粪便样本中为3%。研究结果表明,隐孢子虫感染在印度南部腹泻儿童中很常见,该地区无症状带囊者的比例也很高。