Cotonat A, Cotonat J
Laboratoire de Pharmacologie, C.H.U. Rangueil, Chemin du Vallon, Toulouse, France.
Int Angiol. 1989 Oct-Dec;8(4 Suppl):15-8.
An earlier report proved that Daflon 500 mg constituted of 90% diosmin and 10% hesperidin exerts a lymphagogue effect on dogs. The aim of the present work is to investigate whether the lymphagogue effect of Daflon 500 mg is associated with an increase in pulsatile activity of lymphatic vessels. The investigation was carried out on mongrel dogs anaesthetised by pentobarbital (10 mg/kg); the lymph was collected by a fistula on the thoracic lymphatic duct; using this fistula the pulsatile activity of lymphatic vessels was estimated by Campbell and Health methodology. The lymphatic volume was measured every ten-minutes in graduated tubes for 2 hours and the pulsatile component of intralymphatic pressure (MPC) was estimated from measurements of the area (expressed in mm2) enclosed by the part of the tracing due to the pulsatile component during one minute. Daflon 500 mg was intravenously injected after having been dissolved in DMSO + TRIS; three doses were injected: 12.5, 6.25 and 3.125 mg/kg. Regarding the lymphagogue effect, Daflon 500 mg induced an increase in lymphatic flow correlated with the administered doses. The maximal 10-minute period lymphatic flows were 191% (12.5 mg/kg), 171% (6.25 mg/kg) and 91% (3.125 mg/kg); the peak of the effect was, in each case, reached between 20 and 25 minutes after the injection. Regarding the pulsatile activity, Daflon 500 mg induced an increase of MPC. The MPC was correlated with the increase in lymph flow (r = 0.877).
较早的一份报告证明，由90%地奥司明和10%橙皮苷组成的500毫克微粒化纯化黄酮成分片对犬有促淋巴回流作用。本研究的目的是调查500毫克微粒化纯化黄酮成分片的促淋巴回流作用是否与淋巴管搏动活性增加有关。研究在戊巴比妥（10毫克/千克）麻醉的杂种犬身上进行；通过胸段淋巴管瘘收集淋巴液；使用该瘘管，采用坎贝尔和黑尔思方法评估淋巴管的搏动活性。在刻度管中每10分钟测量一次淋巴液体积，持续2小时，并根据一分钟内由搏动成分引起的描记部分所围成的面积（以平方毫米表示）测量值估算淋巴内压的搏动成分（MPC）。500毫克微粒化纯化黄酮成分片溶于二甲基亚砜+三羟甲基氨基甲烷后静脉注射；注射了三个剂量：12.5、6.25和3.125毫克/千克。关于促淋巴回流作用，500毫克微粒化纯化黄酮成分片可使淋巴液流量增加，且与给药剂量相关。最大10分钟时段的淋巴液流量分别为191%（12.5毫克/千克）、171%（6.25毫克/千克）和91%（3.125毫克/千克）；每种情况下，效应峰值均在注射后20至25分钟达到。关于搏动活性，500毫克微粒化纯化黄酮成分片可使MPC增加。MPC与淋巴液流量增加相关（r = 0.877）。
