Rowji P, Gromkova R, Koornhof H
Emergent Pathogen Research Unit, South African Medical Research Council, School of Pathology, University of Witwatersrand.
J Gen Microbiol. 1989 Oct;135(10):2775-82. doi: 10.1099/00221287-135-10-2775.
Haemophilus influenzae type b strains isolated from children with meningitis, septicaemia and pharyngitis were studied for their ability to undergo genetic transformation by two chromosomal markers, streptomycin resistance and nalidixic acid resistance. Fifty-eight percent of the strains were non-transformable while the remaining 42% showed considerable strain variation with regard to their transformation frequencies, which ranged from 8 x 10(-4) to 1 x 10(-6). The effect of type b capsule on competence development and transformation activity was studied by comparing encapsulated strains with their non-encapsulated variants. Type b capsule did not inhibit either competence development or transforming efficiency. The lack of transformability in the majority of strains was not due to the presence of a capsule.
对从患有脑膜炎、败血症和咽炎的儿童中分离出的b型流感嗜血杆菌菌株，研究了它们通过两个染色体标记物（链霉素抗性和萘啶酸抗性）进行遗传转化的能力。58%的菌株不可转化，而其余42%的菌株在转化频率方面表现出相当大的菌株差异，转化频率范围为8×10⁻⁴至1×10⁻⁶。通过比较包膜菌株与其无包膜变体，研究了b型荚膜对感受态发育和转化活性的影响。b型荚膜既不抑制感受态发育也不抑制转化效率。大多数菌株缺乏可转化性并非由于存在荚膜。
