一种用于测定动物组织中游离肉碱和酯化肉碱的改进型高效液相色谱分析法。
An improved high-performance liquid chromatographic assay for the determination of free and esterified carnitine in animal tissues.
作者信息
Arakawa N, Ha T Y, Otsuka M
机构信息
Department of Food and Nutrition, Ochanomizu University, Tokyo, Japan.
出版信息
J Nutr Sci Vitaminol (Tokyo). 1989 Oct;35(5):475-9. doi: 10.3177/jnsv.35.475.
A high-performance liquid chromatographic procedure for the determination of carnitine that is converted to coenzyme A(CoA) stoichiometrically by carnitine acetyltransferase is described. The chromatographic separation was accomplished by using Unisil QC8 column and a mixture of 190 mM KH2PO4 and methanol (87:13, v/v) as eluent. The present method is rapid, sensitive, and can be used for the routine analysis of animal tissues and plasma.
本文描述了一种高效液相色谱法，用于测定肉碱，该肉碱可通过肉碱乙酰转移酶以化学计量方式转化为辅酶A（CoA）。色谱分离采用Unisil QC8柱，以190 mM KH2PO4和甲醇（87:13，v/v）的混合物作为洗脱剂。本方法快速、灵敏，可用于动物组织和血浆的常规分析。
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