采用高效液相色谱法测定组织提取物中游离肉碱和酯化肉碱的含量。

Measurement of free and esterified carnitine in tissue extracts by high-performance liquid chromatography.

作者信息

Takeyama N, Takagi D, Adachi K, Tanaka T

出版信息

Anal Biochem. 1986 Nov 1;158(2):346-54. doi: 10.1016/0003-2697(86)90559-2.

Abstract

A high-performance liquid chromatographic (HPLC) method for the determination of L-carnitine in clamped and frozen rat livers is described. L-carnitine + acetyl-CoA in equilibrium with acetyl-L-carnitine + CoASH Using the above enzymatic reaction, release of CoASH is stoichiometric with the L-carnitine added. The present method has made possible the determination of carnitine in liver tissues, which is difficult by the conventional enzymatic spectrophotometric method using 5,5'-dithiobis(2-nitrobenzoic acid), owing to acetyl-CoA hydrolysis during prolonged incubations at pH 7.8.

摘要

本文描述了一种用于测定夹闭和冷冻大鼠肝脏中左旋肉碱的高效液相色谱（HPLC）方法。左旋肉碱 + 乙酰辅酶A与乙酰左旋肉碱 + 辅酶A处于平衡状态。利用上述酶促反应，辅酶A的释放与添加的左旋肉碱呈化学计量关系。由于在pH 7.8下长时间孵育期间乙酰辅酶A会水解，传统的使用5,5'-二硫代双（2-硝基苯甲酸）的酶促分光光度法难以测定肝组织中的肉碱，而本方法使得肝组织中肉碱的测定成为可能。