一种用于干血斑中卡马西平、拉莫三嗪和丙戊酸治疗药物监测的液相色谱-串联质谱法。
A LC-MS/MS method for therapeutic drug monitoring of carbamazepine, lamotrigine and valproic acid in DBS.
作者信息
Linder Camilla, Andersson Maria, Wide Katarina, Beck Olof, Pohanka Anton
机构信息
Department of Laboratory Medicine, Clinical Pharmacology, Karolinska Institute, Stockholm, Sweden.
Chemistry and Drug Metabolism section, National Institute on Drug Abuse, National Institute of Health, Baltimore, MD, USA.
出版信息
Bioanalysis. 2015;7(16):2031-9. doi: 10.4155/bio.15.99.
BACKGROUND
Therapeutic drug monitoring of antiepileptic drugs in children with epilepsy assists for personalized drug therapy but require numerous patient visits for venous blood sampling. DBS is an alternative matrix applicable to home sampling which can save time and reduce stress for this patient group.
RESULTS
A fast LC-MS/MS method for quantification of carbamazepine, lamotrigine and valproic acid based on DBS sampling was developed. The method showed linearity in therapeutically relevant concentration ranges and compatible with unknown volume sampling and expected hematocrit range of the patient group.
CONCLUSION
A LC-MS/MS method for the three most commonly used antiepileptic drugs has been fully validated and clinically applied on DBSs from patients at the neuropediatric clinic at Karolinska University Hospital.
背景
对癫痫患儿进行抗癫痫药物的治疗药物监测有助于实现个性化药物治疗，但需要患儿多次就诊进行静脉采血。干血斑是一种适用于家庭采样的替代样本基质，可为该患者群体节省时间并减轻压力。
结果
开发了一种基于干血斑采样的快速液相色谱-串联质谱法，用于定量卡马西平、拉莫三嗪和丙戊酸。该方法在治疗相关浓度范围内呈线性，并且与未知血量采样以及患者群体预期的血细胞比容范围兼容。
结论
一种用于三种最常用抗癫痫药物的液相色谱-串联质谱法已得到充分验证，并已在卡罗林斯卡大学医院神经儿科门诊患者的干血斑样本上进行了临床应用。
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