Johnson Rachel A, Manley Olivia M, Spuches Anne M, Grossoehme Nicholas E
Department of Chemistry, East Carolina University, Greenville, NC 27858, United States.
Department of Chemistry, Physics and Geology, Winthrop University, Rock Hill, SC 29730, United States.
Biochim Biophys Acta. 2016 May;1860(5):892-901. doi: 10.1016/j.bbagen.2015.08.018. Epub 2015 Aug 29.
ITC is a powerful technique that can reliably assess the thermodynamic underpinnings of a wide range of binding events. When metal ions are involved, complications arise in evaluating the data due to unavoidable solution chemistry that includes metal speciation and a variety of linked equilibria.
This paper identifies these concerns, provides recommendations to avoid common mistakes, and guides the reader through the mathematical treatment of ITC data to arrive at a set of thermodynamic state functions that describe identical chemical events and, ideally, are independent of solution conditions. Further, common metal chromophores used in biological metal sensing studies are proposed as a robust system to determine unknown solution competition.
Metal ions present several complications in ITC experiments. This review presents strategies to avoid these pitfalls and proposes and experimentally validates mathematical approaches to deconvolute complex equilibria that exist in these systems.
This review discusses the wide range of complications that exists in metal-based ITC experiments. It provides a starting point for scientists new to this field and articulates concerns that will help experienced researchers troubleshoot experiments.
等温滴定量热法（ITC）是一种强大的技术，能够可靠地评估各种结合事件的热力学基础。当涉及金属离子时，由于不可避免的溶液化学过程（包括金属形态和各种连锁平衡），在评估数据时会出现复杂情况。
本文识别了这些问题，提供了避免常见错误的建议，并引导读者对ITC数据进行数学处理，以得出一组描述相同化学事件且理想情况下与溶液条件无关的热力学状态函数。此外，提出将生物金属传感研究中常用的金属发色团作为确定未知溶液竞争的稳健系统。
金属离子在ITC实验中存在多种复杂情况。本综述提出了避免这些陷阱的策略，并提出并通过实验验证了对这些系统中存在的复杂平衡进行反褶积的数学方法。
本综述讨论了基于金属的ITC实验中存在的各种复杂情况。它为该领域的新手科学家提供了一个起点，并阐明了一些问题，有助于经验丰富的研究人员对实验进行故障排除。
