Wang Maojie, Zhao Jin, Zhao Ya, Huang Run-Yue, Li Geng, Zeng Xing, Li Xiong
a Second Affiliated Hospital , Guangzhou University of Chinese Medicine , Guangzhou , China.
b The Postdoctoral Research Station , Guangzhou University of Chinese Medicine , Guangzhou , China.
Nat Prod Res. 2016 Aug;30(16):1796-801. doi: 10.1080/14786419.2015.1079186. Epub 2015 Sep 1.
One new coumarin, 3,5-dihydroxy-7-O-α-L-rhamno pyranosyl-2H-chromen-2-one (1), was isolated from the whole plant of Sarcandra glabra. The structure was elucidated by spectroscopic methods. Our results indicated that 1 significantly inhibit nitric oxide (NO) production in LPS-induced RAW264.7 macrophages. RT-PCR analysis indicated it inhibited iNOS mRNA expression. In addition, Western blot analysis showed that 1 attenuated LPS-induced synthesis of iNOS protein in the macrophages. These results suggest that 1 could be potential anti-inflammatory agent by down-regulating iNOS expression.
从肿节风全草中分离得到一种新的香豆素，3,5 - 二羟基 - 7 - O - α - L - 鼠李吡喃糖基 - 2H - 色原酮 - 2 - 酮（1）。通过光谱方法对其结构进行了鉴定。我们的结果表明，化合物1能显著抑制脂多糖（LPS）诱导的RAW264.7巨噬细胞中一氧化氮（NO）的产生。逆转录 - 聚合酶链反应（RT - PCR）分析表明它抑制诱导型一氧化氮合酶（iNOS）mRNA的表达。此外，蛋白质免疫印迹分析显示化合物1能减弱LPS诱导的巨噬细胞中iNOS蛋白的合成。这些结果表明化合物1可能是一种通过下调iNOS表达发挥潜在抗炎作用的药物。
