Xu Jing, Xing Xiao-Juan, Tian Yong-Sheng, Peng Ri-He, Xue Yong, Zhao Wei, Yao Quan-Hong
Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.
PLoS One. 2015 Sep 1;10(9):e0136960. doi: 10.1371/journal.pone.0136960. eCollection 2015.
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.
尽管谷胱甘肽S-转移酶（GST，EC 2.5.1.18）参与植物对非生物胁迫的响应，但关于其在番茄中的基因功能的信息有限。在本研究中，克隆了一个来自番茄的GST基因，命名为LeGSTU2，并对其进行了功能鉴定。表达谱分析结果表明，该基因在根和花中表达，其转录受盐、渗透和热胁迫诱导。然后通过根癌农杆菌介导的转化将该基因导入拟南芥。与野生型植物相比，转基因拟南芥植株在生长和成熟方面表现正常。转基因植物对NaCl和甘露醇诱导的盐胁迫和渗透胁迫也表现出增强的抗性。转基因植物耐受性的提高与脯氨酸、丙二醛和抗氧化酶活性的变化相关。我们的结果表明，来自番茄的该基因在提高拟南芥对盐胁迫和干旱胁迫的耐受性方面发挥了积极作用。
