Alpcan Serhan, Başar Halil, Aydos Tolga Reşat, Kul Oğuz, Kısa Üçler, Başar Murad Mehmet
Department of Urology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey.
Department of Pharmacology, Başkent University Faculty of Medicine, Ankara, Turkey.
Turk J Urol. 2014 Dec;40(4):199-206. doi: 10.5152/tud.2014.76892.
We aimed to investigate the changes in endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expression and apoptotic index in rat testicular tissue, as well as serum and seminal plasma sex hormone levels after vasectomy, and the effect of ozone therapy (OT).
Adult male Wistar rats were used (n=6 per group). Control (G1), sham for 4 weeks (G2) or 6 weeks (G3), orchiectomy at the 4(th) (G4) or 6(th) (G5) week after left vasectomy, orchiectomy at the 4(th) (G6) or 6(th) (G7) week after bilateral vasectomy, orchiectomy after 6 weeks OT following left (G8) or bilateral (G9) vasectomy, orchiectomy after 6 weeks OT (G10).
In the left testes, while there were increases in eNOS and iNOS immunoreactivity and apoptotic indexes in G4 and G5, no changes were observed in contralateral testis. These values increased in G6 and G7, while OT inhibited these parameters in the left testis of G8 and both testes of G9. Sex hormone levels did not show any changes after vasectomy and ozone therapy.
While OT was found to be protective against some parameters mentioned above under stress conditions, it seemed to cause some harmful effects when used in healthy conditions.
我们旨在研究输精管结扎术后大鼠睾丸组织中内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）的表达变化、凋亡指数，以及血清和精浆性激素水平，以及臭氧疗法（OT）的作用。
使用成年雄性Wistar大鼠（每组n = 6）。对照组（G1）、假手术4周（G2）或6周（G3）、左侧输精管结扎术后第4周（G4）或第6周（G5）进行睾丸切除术、双侧输精管结扎术后第4周（G6）或第6周（G7）进行睾丸切除术、左侧（G8）或双侧（G9）输精管结扎术后6周OT后进行睾丸切除术、6周OT后进行睾丸切除术（G10）。
在左侧睾丸中，G4和G5组的eNOS和iNOS免疫反应性及凋亡指数增加，而对侧睾丸未观察到变化。这些值在G6和G7组中增加，而OT在G8组的左侧睾丸和G9组的双侧睾丸中抑制了这些参数。输精管结扎术和臭氧疗法后性激素水平未显示任何变化。
虽然发现OT在应激条件下对上述某些参数具有保护作用，但在健康条件下使用时似乎会产生一些有害影响。
