Hazar Aydin Ismet, Cakiroglu Basri, Sakalli Ertan, Balci Mustafa Bahadir Can, Eyyupoglu Erkan, Tas Tuncay, Sinanoglu Orhun, Tuzlali Pinar, Cilesiz Nusret Can
Taksim Training and Research Hospital, Department of Urology Istanbul.
Arch Ital Urol Androl. 2015 Sep 30;87(3):198-203. doi: 10.4081/aiua.2015.3.198.
The aim of this study was to enlighten both the testicular histology and the genetic aspects of the apoptotic process. Thus an experimental study was designed with a model of unilateral vasectomy.
Twenty-two adult male rats were used and 4 main groups were formed. The first (A), the second (B), the third (C), and the fourth group (D) consisted of 4, 4, 4 and 10 rats respectively. Rats in group A had sham operation while rats in other groups (B, C, D) underwent left vasectomy operation including binding of ductus deferens with a 3/0 silk and cutting a minimum of 1 cm part while preserving the vascular structure under 9x magnification. Rats undergoing unilateral vasectomy were sacrificed at the 1(st), 2(nd) and 8(th) weeks and their testicular structure and proapoptotic gene proteins were compared with that of the control group undergoing sham operation.
We found that vasectomy gradually caused destruction and both ipsilateral and contralateral testicles were affected showing initial apoptosis.
The procedure causes destruction in the testicular structure by causing bilateral intratubular germ cell necrosis, unilateral obstruction, increase in the tubular pressure and processes that are aggravated by some probable autoimmune reactions.
本研究旨在阐明睾丸组织学以及凋亡过程的遗传学方面。因此，设计了一项采用单侧输精管结扎模型的实验研究。
使用22只成年雄性大鼠，分为4个主要组。第一组（A）、第二组（B）、第三组（C）和第四组（D）分别由4只、4只、4只和10只大鼠组成。A组大鼠进行假手术，而其他组（B、C、D）的大鼠接受左侧输精管结扎手术，包括用3/0丝线结扎输精管，并在9倍放大倍数下保留血管结构的同时切除至少1厘米长的部分。接受单侧输精管结扎的大鼠在第1周、第2周和第8周处死，将其睾丸结构和促凋亡基因蛋白与接受假手术的对照组进行比较。
我们发现输精管结扎逐渐导致破坏，同侧和对侧睾丸均受到影响，出现初始凋亡。
该手术通过引起双侧曲细精管生殖细胞坏死、单侧梗阻、管腔内压力升高以及一些可能的自身免疫反应加重的过程，导致睾丸结构破坏。
