Liu Sheng, Zhang Kai, Xiao Liang
a BGI Education Center, University of Chinese Academy of Sciences , Shenzhen , China and.
b BGI-Shenzhen , Shenzhen , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3711-2. doi: 10.3109/19401736.2015.1079869. Epub 2015 Sep 2.
The fathead minnow, Pimephales promelas, is an ecological important fish distributed across North America. In this study, we firstly assembled the complete mitochondrial genome of P. promelas. The mitogenome is 16 709 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions. The gene content and order is in accord with the common vertebrate form. ATG acts as the start codon for all PCGs except cytochrome c oxidase subunit I (COXI), which has GTG as a start codon. The nucleotide base composition of H-strand is 29.2% A, 27.9% T, 25.9% C, and 17.0% G. The complete mitochondrial genome data would be useful for further studies of reproduction, development, and adaptive evolution of P. promelas.
黑头呆鱼（Pimephales promelas）是一种在北美广泛分布的具有重要生态意义的鱼类。在本研究中，我们首次组装了黑头呆鱼的完整线粒体基因组。该线粒体基因组长度为16709 bp，包括13个蛋白质编码基因（PCGs）、22个转运RNA（tRNA）基因、2个核糖体RNA（rRNA）基因和2个非编码区。基因内容和顺序与常见的脊椎动物形式一致。除细胞色素c氧化酶亚基I（COXI）以GTG作为起始密码子外，所有PCGs均以ATG作为起始密码子。重链的核苷酸碱基组成为29.2%A、27.9%T、25.9%C和17.0%G。完整的线粒体基因组数据将有助于对黑头呆鱼的繁殖、发育和适应性进化进行进一步研究。
