Dou Xiao-Qiu, Zhang Jia, Feng Chuanliang
State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiaotong University , 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.
ACS Appl Mater Interfaces. 2015 Sep 23;7(37):20786-92. doi: 10.1021/acsami.5b05828. Epub 2015 Sep 8.
To promote cell adhesion in three-dimensional (3D) extracellular matrix (ECM) is crucial for avoiding cell anoikis, which is one of the most important issues for fundamental cell biology. Herein, a biotin-avidin based universal cell-matrix interaction for different types of cells is developed in order to achieve the promoted adhesion in 3D ECM. For the purpose, biotinylated nanofibrous hydrogels are constructed by coassembling 1,4-benzyldicarboxamide (C2) based non-biotinylated and biotinylated supramolecular gelators. The used cells are modified by avidin (AV-cells) through biotinylating cells and then interacting with avidin. After in situ encapsulating AV-cells in the hydrogels, the adhered amount can be increased by tens of percent even with adding several percentages of the biotinylated C2 gelators in the coassembly due to the specific biotin-avidin interaction. Reverse transcription polymerase chain reaction (RT-PCR) confirms that AV-cells can proliferate without varying gene expression and denaturation. Compared with the interaction between RGD and cells, this avidin-biotin interaction should be much more universal and it is feasible to be employed to promote cell adhesion for most types of cells in 3D matrix.
促进细胞在三维（3D）细胞外基质（ECM）中的黏附对于避免细胞失巢凋亡至关重要，这是基础细胞生物学中最重要的问题之一。在此，开发了一种基于生物素-抗生物素蛋白的通用细胞-基质相互作用，用于不同类型的细胞，以实现细胞在3D ECM中的促进黏附。为此，通过共组装基于1,4-苄基二羧酰胺（C2）的非生物素化和生物素化超分子凝胶剂构建生物素化纳米纤维水凝胶。所使用的细胞通过对细胞进行生物素化然后与抗生物素蛋白相互作用，用抗生物素蛋白（AV-细胞）进行修饰。在将AV-细胞原位包封在水凝胶中后，由于特异性生物素-抗生物素蛋白相互作用，即使在共组装中添加几个百分点的生物素化C2凝胶剂，黏附量也可以增加几十%。逆转录聚合酶链反应（RT-PCR）证实AV-细胞可以增殖而不改变基因表达和变性。与RGD和细胞之间的相互作用相比，这种抗生物素蛋白-生物素相互作用应该更具通用性，并且用于促进大多数类型细胞在3D基质中的黏附是可行的。
