Worcester Polytechnic Institute , Worcester , Massachusetts 01605 , United States.
Maine Medical Center Research Institute , Scarborough , Maine 04074 , United States.
Biomacromolecules. 2018 Sep 10;19(9):3705-3713. doi: 10.1021/acs.biomac.8b00824. Epub 2018 Aug 8.
Silk fibroin biomaterials are highly versatile in terms of materials formation and functionalization, with applications in tissue engineering and drug delivery, but necessitate modifications for optimized biological activity. Herein, a facile, avidin-based technique is developed to noncovalently functionalize silk materials with bioactive molecules. The ability to adsorb avidin to silk surfaces and subsequently couple biotinylated macromolecules via avidin-biotin interaction is described. This method better preserved functionality than standard covalent coupling techniques using carbodiimide cross-linking chemistry. The controlled release of avidin from the silk surface was demonstrated by altering the adsorption parameters. Application of this technique to culturing human foreskin fibroblasts (hFFs) and human mesenchymal stem cells (hMSCs) on arginine-glycine-aspartic-acid-modified (RGD-modified) silk showed increased cell growth over a seven-day period. This technique provides a facile method for the versatile functionalization of silk materials for biomedical applications including tissue engineering, drug delivery, and biological sensing.
丝素生物材料在材料形成和功能化方面具有高度的多功能性，可应用于组织工程和药物输送，但需要进行修饰以优化生物活性。本文开发了一种简便的基于亲和素的技术，可将生物活性分子非共价功能化到丝素材料上。描述了将亲和素吸附到丝素表面上，然后通过亲和素-生物素相互作用偶联生物素化的大分子的能力。与使用碳二亚胺交联化学的标准共价偶联技术相比，这种方法更好地保留了功能。通过改变吸附参数，证明了亲和素从丝素表面的控制释放。该技术应用于精氨酸-甘氨酸-天冬氨酸修饰（RGD 修饰）丝素上培养人包皮成纤维细胞（hFFs）和人间充质干细胞（hMSCs），结果显示细胞在七天内的生长增加。该技术为丝素材料的多功能功能化提供了一种简便的方法，可用于生物医学应用，包括组织工程、药物输送和生物传感。
