Zhu Tiebing, Yao Qi, Hu Xiaonan, Chen Chen, Yao Honghong, Chao Jie
Cell Physiol Biochem. 2015;37(2):577-91. doi: 10.1159/000430378.
BACKGROUND/AIMS: Monocyte chemotactic protein-induced protein 1 (MCPIP1) plays a crucial role in various cellular processes, including neurogenesis. However, the relationship between MCPIP1 and myocardial ischemia/reperfusion (I/R) injury remained illdefined. In this study, we explored whether the I/R-mediated up-regulation of MCPIP1 is critical in the modulation of both cell migration and apoptosis in human umbilical vein endothelial cells (HUVECs).
Using Western blot analysis and quantitative real-time PCR, the protein expression and mRNA transcription, respectively, of MCPIP1 was detected in HUVECs. To investigate cell migration, an in vitro scratch assay and a nested matrix model were applied.
I/R increased the expression of MCPIP1 via the activation of the mitogen-activated protein kinase (MAPK) and PI3K/Akt pathways. I/R increased migration and apoptosis of HUVECs, which were significantly inhibited by MCPIP1 siRNA. conclusion: These findings suggest that I/R-mediated up-regulation of MCPIP1 regulates migration and apoptosis in HUVECs. Understanding the regulation of MCPIP1 expression and function may aid in the development of an adjunct therapeutic strategy in the treatment of individuals with I/R injury.
背景/目的：单核细胞趋化蛋白诱导蛋白1（MCPIP1）在包括神经发生在内的各种细胞过程中发挥关键作用。然而，MCPIP1与心肌缺血/再灌注（I/R）损伤之间的关系仍不明确。在本研究中，我们探讨了I/R介导的MCPIP1上调在调节人脐静脉内皮细胞（HUVECs）的细胞迁移和凋亡中是否至关重要。
使用蛋白质印迹分析和定量实时PCR分别检测HUVECs中MCPIP1的蛋白质表达和mRNA转录。为了研究细胞迁移，应用了体外划痕试验和巢式基质模型。
I/R通过激活丝裂原活化蛋白激酶（MAPK）和PI3K/Akt途径增加了MCPIP1的表达。I/R增加了HUVECs的迁移和凋亡，而MCPIP1 siRNA显著抑制了这些作用。结论：这些发现表明，I/R介导的MCPIP1上调调节了HUVECs的迁移和凋亡。了解MCPIP1表达和功能的调节可能有助于制定治疗I/R损伤个体的辅助治疗策略。
