Teijeiro Juan Manuel, Roldán María Lorena, Marini Patricia Estela
Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 590, S2002LRK, Rosario, Argentina.
Researcher from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rosario, Argentina.
Cell Tissue Res. 2016 Feb;363(2):567-77. doi: 10.1007/s00441-015-2266-9. Epub 2015 Sep 2.
In many mammals, upon entry into the female reproductive tract, a subpopulation of sperm is stored in the oviduct forming a functional reservoir. In the oviducts of pig and cow, Annexin A2 (AnxA2) has been linked to the binding of sperm. This protein may exist as a monomer or bound to S100A10 and both forms are associated with different biological functions. S100A10 has not yet been reported in the oviduct. The objective of this work is to analyze for the presence of S100A10 in the oviduct and to advance the study of AnxA2 and S100A10 in this organ. This work shows the presence of both proteins, AnxA2 and S100A10, in the oviduct of human, pig, cow, cat, dog and rabbit. At least in pig, AnxA2 is found devoid of S100A10 in the outer surface of the apical plasma membrane of oviductal epithelial cells, indicating that it binds to sperm as a monomer or in association with proteins different from S100A10. In the apical cytoplasm of pig oviductal epithelial cells, AnxA2 is associated with S100A10. In primary culture of porcine oviductal cells, the expression of ANXA2 is increased by progesterone, while the expression of S100A10 is increased by progesterone and estradiol. The widespread detection of both proteins in the oviduct of mammals indicates a probable conserved function in this organ. In summary, S100A10 and AnxA2 are widespread in the mammalian oviduct but AnxA2 binds sperm in vivo devoid of S100A10 and may be related to reservoir formation.
在许多哺乳动物中，精子进入雌性生殖道后，一部分精子会储存在输卵管中形成一个功能性储存库。在猪和牛的输卵管中，膜联蛋白A2（AnxA2）与精子的结合有关。这种蛋白质可能以单体形式存在，也可能与S100A10结合，两种形式都与不同的生物学功能相关。S100A10在输卵管中尚未见报道。本研究的目的是分析输卵管中S100A10的存在情况，并推进对该器官中AnxA2和S100A10的研究。本研究表明，在人、猪、牛、猫、狗和兔子的输卵管中均存在AnxA2和S100A10这两种蛋白质。至少在猪中，在输卵管上皮细胞顶端质膜的外表面发现AnxA2不与S100A10结合，这表明它作为单体或与不同于S100A10的蛋白质结合与精子结合。在猪输卵管上皮细胞的顶端细胞质中，AnxA2与S100A10相关联。在猪输卵管细胞的原代培养中，孕酮可增加ANXA2的表达，而孕酮和雌二醇均可增加S100A10的表达。在哺乳动物输卵管中广泛检测到这两种蛋白质，表明它们在该器官中可能具有保守功能。总之，S100A10和AnxA2在哺乳动物输卵管中广泛存在，但AnxA2在体内不与S100A10结合的情况下与精子结合，可能与储存库的形成有关。
