Sun Tian Yi, Li Lei, Xin Tianrong, Wang Yayu, Xia Bin
a College of Life Science, Nanchang University , Nanchang , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3676-7. doi: 10.3109/19401736.2015.1079851. Epub 2015 Sep 2.
We determined the complete mitochondrial genome (mitogenome) sequence of Cryptolestes ferrugineus (GenBank accession number KT182067) by the long PCR and primer walking method. The mitochondrial genome is a typical circular DNA molecule of 15 511 bp in length, and contains 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a A + T-rich region (D-loop). The order of 37 genes was typical of insect mitochondrial DNA sequences described to date. The base composition of the genome is A (39.17%), T (37.24%), C (14.22%), and G (9.37%) with an A + T-rich hallmark as that of other invertebrate mitochondrial genomes. All protein-coding genes start with ATN codon and terminate with the stop codon T (AA) or TAG. The A + T-rich region is located between 12S rRNA and tRNA(Ile). In this study, the phylogenetic relationships of Coleoptera species were constructed based on the nucleotides sequences of 13 PCGs of mitogenomes. The molecular-based phylogeny supported the traditional morphological classification on relationships within Coleoptera species.
我们通过长链PCR和引物步移法测定了锈赤扁谷盗（Cryptolestes ferrugineus）的完整线粒体基因组（线粒体基因组）序列（GenBank登录号KT182067）。线粒体基因组是一个典型的环状DNA分子，长度为15511 bp，包含13个蛋白质编码基因、两个核糖体RNA基因、22个转运RNA基因和一个富含A+T的区域（D环）。37个基因的排列顺序是迄今为止所描述的昆虫线粒体DNA序列的典型排列。基因组的碱基组成为A（39.17%）、T（37.24%）、C（14.22%）和G（9.37%），具有与其他无脊椎动物线粒体基因组一样的富含A+T的特征。所有蛋白质编码基因均以ATN密码子起始，并以终止密码子T（AA）或TAG终止。富含A+T的区域位于12S rRNA和tRNA（Ile）之间。在本研究中，基于线粒体基因组13个PCG的核苷酸序列构建了鞘翅目物种的系统发育关系。基于分子的系统发育支持了鞘翅目物种间关系的传统形态学分类。
