Flechsig Paul, Kratochwil Clemens, Warth Arne, Rath Daniel, Eichwald Viktoria, Huber Peter E, Kauczor Hans-Ulrich, Haberkorn Uwe, Giesel Frederik L
Department of Diagnostic and Interventional Radiology, University Hospital Heidelberg, Heidelberg, Germany.
Translational Lung Research Center Heidelberg, Member of the German Center for Lung Research DZL, Heidelberg, Germany.
Mol Imaging Biol. 2016 Apr;18(2):243-8. doi: 10.1007/s11307-015-0890-0.
The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats.
Human lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG). Thereafter, animals were sacrificed for histological analysis.
Late phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [(18)F]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases.
The combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases.
优化肺部及其他器官良恶性疾病患者多学科治疗策略的需求，导致对使用新型非侵入性成像方法的临床前小动物模型进行机制性概念验证研究的需求增加。因此，我们评估了微型正电子发射断层扫描（microPET）和微型计算机断层扫描（microCT）在大鼠原位肺癌模型纵隔淋巴结分期中的作用。
将人肺癌细胞（NCI-H460）经胸注射到裸鼠（NIH-RNU）体内。肿瘤生长2周后，动物接受使用ExiTron nano 12000作为造影剂的多期增强微型计算机断层扫描（microCT）以及使用示踪剂2-脱氧-2-[(18)F]氟-D-葡萄糖（[(18)F]FDG）的动态微型正电子发射断层扫描（microPET）。此后，处死动物进行组织学分析。
与早期扫描相比，晚期微型X射线计算机断层扫描（microCT）对淋巴结转移的显示最佳。就[(18)F]FDG摄取随时间增加而言，动态微型正电子发射断层扫描（microPET）可显示淋巴结转移，并能够对诱发的肺癌转移进行代谢检查。
在大鼠中，增强微型计算机断层扫描（microCT）和动态微型正电子发射断层扫描（microPET）相结合对于纵隔淋巴结转移的可视化是可行的。
