将封装蛋白笼纳米颗粒基因工程化为 SCC-7 细胞靶向光学纳米探针。
Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe.
机构信息
Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 689-798 South Korea.
出版信息
Biomater Res. 2014 Dec 23;18:21. doi: 10.1186/2055-7124-18-21. eCollection 2014.
BACKGROUND
Protein cage nanoparticles are promising nanoplatform candidates for efficient delivery systems of diagnostics and/or therapeutics because of their uniform size and structure as well as high biocompatibility and biodegradability. Encapsulin protein cage nanoparticle is used to develop a cell-specific targeting optical nanoprobe.
RESULTS
FcBPs are genetically inserted and successfully displayed on the surface of encapsulin to form FcBP-encapsulin. Selectively binding of FcBP-encapsulin to SCC-7 is visualized with fluorescent microscopy.
CONCLUSIONS
Encapsulin protein cage nanoparticle is robust enough to maintain their structure at high temperature and easily acquires multifunctions on demand through the combination of genetic and chemical modifications.
背景
蛋白笼纳米颗粒因其均匀的尺寸和结构以及较高的生物相容性和可生物降解性，成为高效递药系统的诊断和/或治疗学的有前途的纳米平台候选物。包被蛋白纳米颗粒被用于开发细胞特异性靶向光学纳米探针。
结果
FcBPs 被基因插入并成功展示在包被蛋白的表面，形成 FcBP-包被蛋白。荧光显微镜观察到 FcBP-包被蛋白对 SCC-7 的选择性结合。
结论
包被蛋白纳米颗粒足够坚固，能够在高温下保持其结构，并且通过遗传和化学修饰的结合，很容易按需获得多种功能。
Zotero 插件