Hall J B, Chou S T, Louis C J
Department of Pathology, Repatriation General Hospital, Heidelberg, Victoria.
Pathology. 1989 Oct;21(4):239-47. doi: 10.3109/00313028909061067.
The indirect immunoperoxidase technique has been used to demonstrate Lea and Leb antigens in paraffin sections of both morphologically normal gastric and colonic mucosae and their neoplastic counterparts. Expression differed in various regions of the gastrointestinal tract: Leb occurred most frequently in the stomach and Lea most frequently in the colon. Coexpression of Lea and Leb occurred in only 5% of cases of normal mucosa, in 65% of gastric carcinomas and in 82% of carcinomas of the colon. Furthermore, 75% of cases of intestinal metaplasia in gastric mucosa and 30% of tubular adenomas, 50% of villous adenomas and 70% of tubulovillous adenomas in the colon co-expressed Lea and Leb antigens. In this study, the expression of Lewis antigens in carcinoma was found to differ from that of adjacent normal mucosa in 95% of cases of gastric carcinoma and 100% of cases of colonic carcinoma. The differences were shown by antigen acquisition and/or deletion. Similar changes were shown in 88% of cases of intestinal metaplasia in gastric mucosa, 20% of cases of tubular adenomas and 57% of cases of villous and tubulovillous adenomas of colon.
间接免疫过氧化物酶技术已用于在形态学正常的胃和结肠黏膜及其肿瘤对应组织的石蜡切片中显示Lea和Leb抗原。胃肠道不同区域的表达有所不同：Leb最常见于胃，而Lea最常见于结肠。Lea和Leb的共表达仅发生在5%的正常黏膜病例、65%的胃癌病例和82%的结肠癌病例中。此外，胃黏膜肠化生病例的75%以及结肠管状腺瘤的30%、绒毛状腺瘤的50%和管状绒毛状腺瘤的70%共表达Lea和Leb抗原。在本研究中，发现95%的胃癌病例和100%的结肠癌病例中癌组织中Lewis抗原的表达与相邻正常黏膜不同。这些差异通过抗原获得和/或缺失表现出来。88%的胃黏膜肠化生病例、20%的管状腺瘤病例以及57%的结肠绒毛状和管状绒毛状腺瘤病例也显示出类似变化。
