Norazmi M, Hohmann A W, Skinner J M, Bradley J
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
Pathology. 1989 Oct;21(4):248-53. doi: 10.3109/00313028909061068.
Malignant and non-malignant ('normal') colonic tissues from patients with colonic carcinoma were examined for the expression of MHC class I and class II antigens by immunoenzymatic staining using monoclonal antibodies. The amount of class I antigen as detected by 2 monoclonal antibodies, FMC 16 or W6/32 was clearly diminished in 11 of 14 tumours when compared to the amount present on 'normal' colonic tissue from the same individual. The loss of class I antigen did not correlate with tumour stage or differentiation. The reactivities of FMC 16 and W6/32 with these tissues were not identical, which indicates that the 2 monoclonal antibodies may recognize different epitopes on the HLA class I molecule. Class II antigens were absent from 'normal' colonic epithelium but were present on 20 of 28 tumours, with DR being detected more often than DP, and DQ found only on 4 of 28 tumours. When present, staining for class II antigens was heterogeneous within the tumour, in that all tumour cells did not stain equally. DR and DP antigens were found more often on moderately or poorly differentiated adenocarcinomas and on stage B, C and D tumours in that order of frequency. Thus tumours with a better prognosis were less likely to express DR and DP. The expression of DQ was unrelated to staging or differentiation.
利用单克隆抗体通过免疫酶染色法检测结肠癌患者的恶性和非恶性（“正常”）结肠组织中MHC I类和II类抗原的表达。与来自同一个体的“正常”结肠组织相比，在14个肿瘤中的11个中，用两种单克隆抗体FMC 16或W6/32检测到的I类抗原量明显减少。I类抗原的缺失与肿瘤分期或分化无关。FMC 16和W6/32与这些组织的反应性并不相同，这表明这两种单克隆抗体可能识别HLA I类分子上不同的表位。“正常”结肠上皮中不存在II类抗原，但在28个肿瘤中的20个中存在，检测到DR的频率高于DP，而DQ仅在28个肿瘤中的4个中发现。当存在时，肿瘤内II类抗原的染色是异质性的，即并非所有肿瘤细胞都有同等程度的染色。DR和DP抗原更常见于中分化或低分化腺癌以及B、C和D期肿瘤，频率依次降低。因此，预后较好的肿瘤表达DR和DP的可能性较小。DQ的表达与分期或分化无关。
