Michalevicz R, Lifshitz D, Revel M
Hematology Institute, Ichilov Hospital, Tel Aviv, Israel.
Scanning Microsc. 1989 Dec;3(4):1143-9; discussion 1149-50.
Early 4-hydroxyperoxycyclophosphamide (4-HC) resistant hematopoietic progenitor cells (pre-colony-forming units, pre-CFU) were evaluated by a two-step liquid culture system, (earlier progenitors), pre-CFU, as well as by the conventional semi-solid mixed colony assay (later progenitors) for their growth response to interleukin-6 (IL-6), interleukin-3 (IL-3), and a combination of both factors. The effect of the IL-6/IL-3 combination was compared to that of IL-1/IL-3. IL-3 alone proved less effective in supporting earlier pre-CFU cells than later progenitor cells. In a previous work IL-6 promoted the growth of early multipotential progenitor cells circulating in hairy cell leukemia (HCL) patients. IL-6 alone did not stimulate growth of either early or later normal progenitor cells. However, a significant synergistic effect was obtained when IL-6 and IL-3 were added together (p less than 0.05). IL-6/IL-3 synergism was more potent than IL-1/IL-3 in promoting growth of colonies. The previously described synergistic effect of IL-1/IL-3 seems to be independent of IL-6. Thus, our results suggest that the multi-functional cytokine IL-6, may be of use in shortening the engraftment time in bone marrow transplantation.
通过两步液体培养系统评估早期对4-羟基过氧环磷酰胺（4-HC）耐药的造血祖细胞（集落形成前单位，pre-CFU），以及通过传统的半固体混合集落测定法评估晚期祖细胞，观察它们对白细胞介素-6（IL-6）、白细胞介素-3（IL-3）以及这两种因子组合的生长反应。将IL-6/IL-3组合的效果与IL-1/IL-3的效果进行比较。单独的IL-3在支持早期pre-CFU细胞方面比晚期祖细胞效果更差。在之前的一项研究中，IL-6促进了毛细胞白血病（HCL）患者循环中的早期多能祖细胞的生长。单独的IL-6既不刺激早期也不刺激晚期正常祖细胞的生长。然而，当IL-6和IL-3一起添加时，获得了显著的协同效应（p小于0.05）。在促进集落生长方面，IL-6/IL-3的协同作用比IL-1/IL-3更强。之前描述的IL-1/IL-3的协同效应似乎与IL-6无关。因此，我们的结果表明，多功能细胞因子IL-6可能有助于缩短骨髓移植中的植入时间。
