de Saint-Georges L, Miller S C, Bowman B M, Jee W S
Division of Radiobiology, School of Medicine, University of Utah, Salt Lake City 84112.
Scanning Microsc. 1989 Dec;3(4):1201-5; discussion 1205-6.
The morphology of in situ osteoclasts on endocortical surfaces of the femoral midshaft was examined by scanning electron microscopy. Mice were perfusion fixed and bone marrow plugs were flushed out of femoral diaphyseal cylinders. The bones were split longitudinally and the endocortical surfaces examined. This method left on the bone surface most of the endosteal cells in their natural, in situ shape and position. Most of the bone surface was lined by contiguous bone lining cells covering resting bone surfaces, making a clear physical barrier between the bone and marrow compartments. On resorption surfaces, which were characterized by excavation cavities, osteoclasts were very polymorphic and spread on the bone surface, extending large pseudopods. The in vivo morphology of individual osteoclasts appears somewhat similar to that described by other investigators on calvaria surfaces and for isolated osteoclasts adherent to artificial substrates. In the resorption domains, osteoclasts appeared to be connected with adjacent osteoclasts, suggesting that the cells form a functional syncytium in resorption areas.
通过扫描电子显微镜检查股骨中轴骨内膜表面原位破骨细胞的形态。小鼠经灌注固定后，将骨髓栓从股骨干骺端圆柱体中冲洗出来。将骨头纵向劈开并检查骨内膜表面。这种方法使大部分骨内膜细胞以其自然的原位形状和位置留在骨表面。大部分骨表面由覆盖静止骨表面的连续骨衬细胞排列，在骨和骨髓腔之间形成了清晰的物理屏障。在以挖掘腔为特征的吸收表面上，破骨细胞形态非常多样，散布在骨表面，伸出大的伪足。单个破骨细胞的体内形态似乎与其他研究者在颅骨表面以及附着在人工基质上的分离破骨细胞所描述的形态有些相似。在吸收区域，破骨细胞似乎与相邻的破骨细胞相连，这表明这些细胞在吸收区域形成了功能性合胞体。
