Seo Ho Bin, Kwon Young Seop, Lee Ji-eun, Cullen David, Noh Hongseok Moses, Gu Man Bock
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea.
Analyst. 2015 Oct 7;140(19):6671-5. doi: 10.1039/c5an00726g.
We present a novel reflectance-based colorimetric aptasensor using gold nanoparticles for the detection of oxytetracycline for the first time. It was found that the reflectance-based measurement at two wavelengths (650 and 520 nm) can generate more stable and sensitive signals than absorbance-based sensors to determine the aggregation of AuNPs, even at high AuNP concentrations. One of the most common antibacterial agents, oxytetracycline (OTC), was detected at concentrations as low as 1 nM in both buffer solution and tap water, which was 25-fold more sensitive, compared to the previous absorbance-based colorimetric aptasensors. This reflectance-based colorimetric aptasensor using gold nanoparticles is considered to be a better platform for portable sensing of small molecules using aptamers.
我们首次展示了一种基于反射率的比色适体传感器，该传感器使用金纳米颗粒来检测土霉素。研究发现，基于反射率在两个波长（650和520纳米）处进行测量，与基于吸光度的传感器相比，即使在高金纳米颗粒浓度下，也能产生更稳定、更灵敏的信号来确定金纳米颗粒的聚集情况。作为最常见的抗菌剂之一，土霉素（OTC）在缓冲溶液和自来水中的检测浓度低至1纳摩尔，与之前基于吸光度的比色适体传感器相比，灵敏度提高了25倍。这种使用金纳米颗粒的基于反射率的比色适体传感器被认为是使用适体对小分子进行便携式传感的更好平台。
