Boothe Emily, Medeiros Matthew C I, Kitron Uriel D, Brawn Jeffrey D, Ruiz Marilyn O, Goldberg Tony L, Walker Edward D, Hamer Gabriel L
Department of Entomology, Texas A&M University, 2475 TAMU, College Station, TX 77843-2475.
Department of Biology, University of Missouri-St. Louis, St. Louis, One University Blvd., MO 63121.
J Med Entomol. 2015 May;52(3):461-8. doi: 10.1093/jme/tjv029. Epub 2015 Mar 31.
Multiple mosquito-borne parasites cocirculate in nature and potentially interact. To understand the community of parasites cocirculating with West Nile virus (WNV), we screened the bloodmeal content of Culex pipiens L. mosquitoes for three common types of hemoparasites. Blood-fed Cx. pipiens were collected from a WNV-epidemic area in suburban Chicago, IL, from May to September 2005 through 2010. DNA was extracted from dissected abdomens and subject to PCR and direct sequencing to identify the vertebrate host. RNA was extracted from the head or thorax and screened for WNV using quantitative reverse transcriptase PCR. Seventy-nine engorged females with avian host origin were screened using PCR and amplicon sequencing for filarioid nematodes, Haemosporida, and trypanosomatids. Filarioid nematodes were identified in 3.8% of the blooded abdomens, Plasmodium sp. in 8.9%, Haemoproteus in 31.6%, and Trypanosoma sp. in 6.3%. The sequences from these hemoparasite lineages were highly similar to sequences from birds in prior studies in suburban Chicago. Overall, 50.6% of blood-fed Culex pipiens contained hemoparasite DNA in their abdomen, presumably from current or prior bloodmeals. Additionally, we detected hemoparasite DNA in the blooded abdomen of three of 10 Cx. pipiens infected with WNV.
多种蚊媒寄生虫在自然界中共同传播并可能相互作用。为了解与西尼罗河病毒（WNV）共同传播的寄生虫群落，我们对致倦库蚊的血餐内容物进行了三种常见血寄生虫类型的筛查。2005年至2010年5月至9月期间，从伊利诺伊州芝加哥郊区的一个WNV流行区采集了吸食血液的致倦库蚊。从解剖的腹部提取DNA，进行PCR和直接测序以鉴定脊椎动物宿主。从头部或胸部提取RNA，使用定量逆转录PCR筛查WNV。对79只来自鸟类宿主的饱血雌蚊进行PCR和扩增子测序，以检测丝状线虫、血孢子虫和锥虫。在3.8%的吸血腹部中鉴定出丝状线虫，疟原虫属占8.9%，血变原虫占31.6%，锥虫属占6.3%。这些血寄生虫谱系的序列与芝加哥郊区先前研究中鸟类的序列高度相似。总体而言，50.6%的吸食血液的致倦库蚊腹部含有血寄生虫DNA，推测来自当前或先前的血餐。此外，在10只感染WNV的致倦库蚊中，有3只的吸血腹部检测到血寄生虫DNA。
