Grzybowski Marcin M, Dziadek Bożena, Gatkowska Justyna M, Dzitko Katarzyna, Długońska Henryka
Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland.
Parasitol Res. 2015 Dec;114(12):4553-63. doi: 10.1007/s00436-015-4701-y. Epub 2015 Sep 4.
Toxoplasmosis is one of the most common parasitic infections worldwide. An effective vaccine against human and animal toxoplasmosis is still needed to control this parasitosis. The polymorphic rhoptry proteins, ROP5 and ROP18, secreted by Toxoplasma gondii during the invasion of the host cell have been recently considered as promising vaccine antigens, as they appear to be the major determinants of T. gondii virulence in mice. The goal of this study was to evaluate their immunogenic and immunoprotective activity after their administration (separately or both recombinant proteins together) with the poly I:C as an adjuvant. Immunization of BALB/c and C3H/HeOuJ mice generated both cellular and humoral specific immune responses with some predominance of IgG1 antibodies. The spleen cells derived from vaccinated animals reacted to the parasite's native antigens. Furthermore, the immunization led to a partial protection against acute and chronic toxoplasmosis. These findings confirm the previous assumptions about ROP5 and ROP18 antigens as valuable components of a subunit vaccine against toxoplasmosis.
弓形虫病是全球最常见的寄生虫感染之一。仍需要一种有效的针对人类和动物弓形虫病的疫苗来控制这种寄生虫病。刚地弓形虫在侵入宿主细胞过程中分泌的多态性棒状体蛋白ROP5和ROP18，最近被认为是有前景的疫苗抗原，因为它们似乎是刚地弓形虫在小鼠中毒力的主要决定因素。本研究的目的是评估在将它们（单独或两种重组蛋白一起）与聚肌胞苷酸作为佐剂一起给药后它们的免疫原性和免疫保护活性。用其免疫BALB/c和C3H/HeOuJ小鼠产生了细胞和体液特异性免疫反应，其中IgG1抗体有一定优势。来自接种疫苗动物的脾细胞对寄生虫的天然抗原有反应。此外，免疫导致对急性和慢性弓形虫病有部分保护作用。这些发现证实了之前关于ROP5和ROP18抗原作为抗弓形虫病亚单位疫苗有价值成分的假设。
